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1n7f

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(New page: 200px<br /> <applet load="1n7f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n7f, resolution 1.80&Aring;" /> '''Crystal structure o...)
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[[Image:1n7f.gif|left|200px]]<br />
 
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<applet load="1n7f" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n7f, resolution 1.80&Aring;" />
 
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'''Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide'''<br />
 
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==Overview==
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==Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide==
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PDZ domains bind to short segments within target proteins in a, sequence-specific fashion. Glutamate receptor-interacting protein, (GRIP)/ABP family proteins contain six to seven PDZ domains and interact, via the sixth PDZ domain (class II) with the C termini of various proteins, including liprin-alpha. In addition the PDZ456 domain mediates the, formation of homo- and heteromultimers of GRIP proteins. To better, understand the structural basis of peptide recognition by a class II PDZ, domain and PDZ-mediated multimerization, we determined the crystal, structures of the GRIP1 PDZ6 domain alone and in complex with a synthetic, C-terminal octapeptide of human liprin-alpha at resolutions of 1.5 and 1.8, A, respectively. Remarkably, unlike other class II PDZ domains, Ile-736 at, alphaB5 rather than conserved Leu-732 at alphaB1 makes a direct, hydrophobic contact with the side chain of the Tyr at the -2 position of, the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight, reorientation of helix alphaB, indicating that the second hydrophobic, pocket undergoes a conformational adaptation to accommodate the bulkiness, of the Tyr side chain, and forms an antiparallel dimer through an, interface located at a site distal to the peptide-binding groove. This, configuration may enable formation of GRIP multimers and efficient, clustering of GRIP-binding proteins.
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<StructureSection load='1n7f' size='340' side='right'caption='[[1n7f]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1n7f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N7F FirstGlance]. <br>
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1N7F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N7F OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7f OCA], [https://pdbe.org/1n7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n7f RCSB], [https://www.ebi.ac.uk/pdbsum/1n7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7f ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of GRIP1 PDZ6-peptide complex reveals the structural basis for class II PDZ target recognition and PDZ domain-mediated multimerization., Im YJ, Park SH, Rho SH, Lee JH, Kang GB, Sheng M, Kim E, Eom SH, J Biol Chem. 2003 Mar 7;278(10):8501-7. Epub 2002 Dec 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12493751 12493751]
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== Function ==
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[[Category: Protein complex]]
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[https://www.uniprot.org/uniprot/LIPA1_HUMAN LIPA1_HUMAN] May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates.<ref>PMID:7796809</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/1n7f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n7f ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Eom, S.H.]]
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[[Category: Eom SH]]
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[[Category: Im, Y.J.]]
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[[Category: Im YJ]]
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[[Category: Kang, G.B.]]
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[[Category: Kang GB]]
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[[Category: Kim, E.]]
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[[Category: Kim E]]
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[[Category: Lee, J.H.]]
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[[Category: Lee JH]]
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[[Category: Park, S.H.]]
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[[Category: Park SH]]
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[[Category: Rho, S.H.]]
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[[Category: Rho SH]]
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[[Category: Sheng, M.]]
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[[Category: Sheng M]]
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[[Category: grip]]
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[[Category: liprin]]
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[[Category: pdz]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:18:44 2007''
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Current revision

Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide

PDB ID 1n7f

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