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1pk8

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[[Image:1pk8.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Rat Synapsin I C Domain Complexed to Ca.ATP==
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|PDB= 1pk8 |SIZE=350|CAPTION= <scene name='initialview01'>1pk8</scene>, resolution 2.1&Aring;
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<StructureSection load='1pk8' size='340' side='right'caption='[[1pk8]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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<table><tr><td colspan='2'>[[1pk8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PK8 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE= SYN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pk8 OCA], [https://pdbe.org/1pk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pk8 RCSB], [https://www.ebi.ac.uk/pdbsum/1pk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pk8 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1aux|1AUX]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pk8 OCA], [http://www.ebi.ac.uk/pdbsum/1pk8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pk8 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/SYN1_RAT SYN1_RAT] Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pk/1pk8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pk8 ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Rat Synapsin I C Domain Complexed to Ca.ATP'''
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==See Also==
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*[[Synapsin|Synapsin]]
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__TOC__
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==Overview==
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</StructureSection>
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Synapsins are multidomain proteins that are critical for regulating neurotransmitter release in vertebrates. In the present study, two crystal structures of the C domain of rat synapsin I (rSynI-C) in complex with Ca(2+) and ATP reveal that this protein can form a tetramer and that a flexible loop (the "multifunctional loop") contacts bound ATP. Further experiments were carried out on a protein comprising the A, B, and C domains of rat synapsin I (rSynI-ABC). An ATP-stabilized tetramer of rSynI-ABC is observed during velocity sedimentation and size-exclusion chromatographic experiments. These hydrodynamic results also indicate that the A and B domains exist in an extended conformation. Calorimetric measurements of ATP binding to wild-type and mutant rSynI-ABC demonstrate that the multifunctional loop and a cross-tetramer contact are important for ATP binding. The evidence supports a view of synapsin I as an ATP-utilizing, tetrameric protein made up of monomers that have a flexible, extended N terminus.
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[[Category: Large Structures]]
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==About this Structure==
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1PK8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PK8 OCA].
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==Reference==
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Tetramerization and ATP binding by a protein comprising the A, B, and C domains of rat synapsin I., Brautigam CA, Chelliah Y, Deisenhofer J, J Biol Chem. 2004 Mar 19;279(12):11948-56. Epub 2003 Dec 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14688264 14688264]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Brautigam CA]]
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[[Category: Brautigam, C A.]]
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[[Category: Chelliah Y]]
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[[Category: Chelliah, Y.]]
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[[Category: Deisenhofer J]]
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[[Category: Deisenhofer, J.]]
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[[Category: atp binding]]
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[[Category: atp grasp]]
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[[Category: calcium (ii) ion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:00:51 2008''
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Current revision

Crystal Structure of Rat Synapsin I C Domain Complexed to Ca.ATP

PDB ID 1pk8

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