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1u9o

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(New page: 200px<br /><applet load="1u9o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u9o, resolution 3.3&Aring;" /> '''Crystal structure of ...)
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[[Image:1u9o.gif|left|200px]]<br /><applet load="1u9o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1u9o, resolution 3.3&Aring;" />
 
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'''Crystal structure of the transcriptional regulator EthR in a ligand bound conformation'''<br />
 
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==Overview==
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==Crystal structure of the transcriptional regulator EthR in a ligand bound conformation==
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Mycobacterium tuberculosis EthR is a repressor of ethA, a gene encoding a, mono-oxygenase required for the activation of the prodrug ethionamide., Here we describe the X-ray crystal structure of EthR, a homodimer with an, entirely helical structure showing similarities to TetR family members., Each monomer contained a fortuitous ligand identified as hexadecyl, octanoate. The crystal structure of EthR purified in M. smegmatis revealed, the presence of a comparable ligand. The binding of hexadecyl octanoate to, EthR induces a conformational state incompatible with repressor function, which should lead to ethA derepression and consequently to an increased, sensitivity to ethionamide and other thioamides. A related, more, hydrophilic ketone was found to exhibit synergistic antimycobacterial, effects when tested together with ethionamide, indicating that this, strategy may help reduce the dosage of potent antibacterial compounds that, otherwise are too toxic to be used as first-line drugs.
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<StructureSection load='1u9o' size='340' side='right'caption='[[1u9o]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1u9o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U9O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CNS:HEXADECYL+OCTANOATE'>CNS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u9o OCA], [https://pdbe.org/1u9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u9o RCSB], [https://www.ebi.ac.uk/pdbsum/1u9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u9o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ETHR_MYCTU ETHR_MYCTU] Involved in the repression of the monooxygenase EthA which is responsible of the formation of the active metabolite of ethionamide (ETH).<ref>PMID:10869356</ref> <ref>PMID:10944230</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u9/1u9o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u9o ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1U9O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with CNS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U9O OCA].
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*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of EthR in a ligand bound conformation reveals therapeutic perspectives against tuberculosis., Frenois F, Engohang-Ndong J, Locht C, Baulard AR, Villeret V, Mol Cell. 2004 Oct 22;16(2):301-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15494316 15494316]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Protein complex]]
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[[Category: Baulard AR]]
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[[Category: Baulard, A.R.]]
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[[Category: Engohang-Ndong J]]
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[[Category: Engohang-Ndong, J.]]
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[[Category: Frenois F]]
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[[Category: Frenois, F.]]
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[[Category: Locht C]]
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[[Category: Locht, C.]]
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[[Category: Villeret V]]
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[[Category: Villeret, V.]]
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[[Category: CNS]]
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[[Category: protein-ligand complex]]
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[[Category: tetr family]]
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[[Category: transcriptional repressor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:34:54 2007''
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Current revision

Crystal structure of the transcriptional regulator EthR in a ligand bound conformation

PDB ID 1u9o

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