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1wn5

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Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Cacodylic Acid

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-[[Image:1wn5.gif|left|200px]]<br /><applet load="1wn5" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1wn5, resolution 1.80&Aring;" />
-'''Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Cacodylic Acid'''<br />
-==Overview==
+==Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Cacodylic Acid==
-Blasticidin S deaminase from Aspergillus terreus was crystallized with, polyethylene glycol 8000. Two types of crystals were grown under the same, crystallization conditions. One type grew as thin plates, while the other, had a rhombic shape. The rhombic shaped crystal was suitable for, high-resolution crystal structure analysis. Precession photographs and, diffraction data showed that the crystal belonged to orthorhombic space, group P212121, with unit-cell dimensions a = 70.33, b = 146.56 and c =, 56.48 A. The calculated Vm value was acceptable when a tetramer of the, enzyme was contained in an asymmetric unit. Preliminary diffraction data, were collected to a resolution of 2.0 A with good statistics.
+<StructureSection load='1wn5' size='340' side='right'caption='[[1wn5]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1wn5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WN5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WN5 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wn5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wn5 OCA], [https://pdbe.org/1wn5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wn5 RCSB], [https://www.ebi.ac.uk/pdbsum/1wn5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wn5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/BSD_ASPTE BSD_ASPTE] Catalyzes the deamination of the cytosine moiety of the antibiotics blasticidin S, cytomycin and acetylblasticidin S.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/1wn5_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wn5 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-WN5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus] with ZN and CAC as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Blasticidin-S_deaminase Blasticidin-S deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.23 3.5.4.23] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WN5 OCA].
+*[[Deaminase 3D structures|Deaminase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus., Nakasako M, Kimura M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):547-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089374 10089374]
 [[Category: Aspergillus terreus]]
-[[Category: Blasticidin-S deaminase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Furuichi M]]
-[[Category: Furuichi, M.]]
+[[Category: Kimura M]]
-[[Category: Kimura, M.]]
+[[Category: Kumasaka T]]
-[[Category: Kumasaka, T.]]
+[[Category: Nakasako M]]
-[[Category: Nakasako, M.]]
+[[Category: Ueki T]]
-[[Category: Ueki, T.]]
+[[Category: Yamaguchi I]]
-[[Category: Yamaguchi, I.]]
+[[Category: Yamamoto M]]
-[[Category: Yamamoto, M.]]
-[[Category: CAC]]
-[[Category: ZN]]
-[[Category: cytidine deaminase family]]
-[[Category: hydrolase]]
-[[Category: tetramer]]
-[[Category: zinc]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:32:28 2007''

1wn5

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Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Cacodylic Acid

Structural highlights

Function

Evolutionary Conservation

See Also

Contents

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