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1wno

From Proteopedia

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[[Image:1wno.gif|left|200px]]
 
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{{Structure
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==Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407==
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|PDB= 1wno |SIZE=350|CAPTION= <scene name='initialview01'>1wno</scene>, resolution 2.10&Aring;
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<StructureSection load='1wno' size='340' side='right'caption='[[1wno]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
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<table><tr><td colspan='2'>[[1wno]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WNO FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wno OCA], [https://pdbe.org/1wno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wno RCSB], [https://www.ebi.ac.uk/pdbsum/1wno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wno ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHIB1_ASPFM CHIB1_ASPFM] Major secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Plays a role in the morphogenesis and autolysis (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/1wno_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wno ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407'''
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==See Also==
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*[[Chitinase 3D structures|Chitinase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Chitinase hydrolyzes chitin, a linear polymer of beta-1,4-linked N-acetylglucosamine (NAG), and plays a variety of roles in the biological world. In addition to endo- and exo-hydrolytic activities, transglycosyl activity has also been observed in the extracellular chitinase (afCHI) from the airborne saprophytic fungi Aspergillus fumigatus YJ-407. Crystals of this native chitinase have been grown at 291 K using PEG 3350 as a precipitant. The diffraction data from the crystal extend to 1.7 A resolution at BSRF, China. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 95.7, b = 100.5, c = 134.3 A. The presence of two molecules per asymmetric unit gives a crystal volume per protein mass (V(M)) of 3.6 A(3) Da(-1) and a solvent content of 65% by volume. A full set of X-ray diffraction data was collected to 2.1 A resolution.
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==About this Structure==
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1WNO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNO OCA].
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==Reference==
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Crystallization and preliminary crystallographic analysis of a native chitinase from the fungal pathogen Aspergillus fumigatus YJ-407., Hu H, Wang G, Yang H, Zhou J, Mo L, Yang K, Jin C, Jin C, Rao Z, Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):939-40. Epub 2004, Apr 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15103145 15103145]
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[[Category: Aspergillus fumigatus]]
[[Category: Aspergillus fumigatus]]
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[[Category: Chitinase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Hu H]]
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[[Category: Hu, H.]]
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[[Category: Jin C]]
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[[Category: Jin, C.]]
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[[Category: Mo L]]
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[[Category: Mo, L.]]
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[[Category: Rao Z]]
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[[Category: Rao, Z.]]
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[[Category: Wang G]]
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[[Category: Wang, G.]]
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[[Category: Yang H]]
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[[Category: Yang, H.]]
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[[Category: Yang K]]
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[[Category: Yang, K.]]
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[[Category: Zhou J]]
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[[Category: Zhou, J.]]
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[[Category: MG]]
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[[Category: NAG]]
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[[Category: NDG]]
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[[Category: SO4]]
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[[Category: eight-stranded beta/alpha-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:59:20 2008''
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Current revision

Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407

PDB ID 1wno

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