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1ww7

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(New page: 200px<br /><applet load="1ww7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ww7, resolution 1.90&Aring;" /> '''Agrocybe cylindracea...)
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[[Image:1ww7.gif|left|200px]]<br /><applet load="1ww7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ww7, resolution 1.90&Aring;" />
 
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'''Agrocybe cylindracea galectin (Ligand-free)'''<br />
 
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==Overview==
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==Agrocybe cylindracea galectin (Ligand-free)==
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Galectin from an edible fungus Agrocybe cylindracea (ACG) has a strong, preference for N-acetylneuraminyl lactose (NeuAcalpha2-3lactose). The, sugar recognition mechanism of ACG was explored by the X-ray, crystallographic analyses of ligand-free ACG, and its complex with, lactose, 3'-sulfonyl lactose and NeuAcalpha2-3lactose. The refined, structure shows that ACG is a "proto"-type galectin composed of a, beta-sandwich of two antiparallel sheets, each with six strands, in, contrast to the five and six strands in animal galectins. ACG dimer in, solution was classified as being among the "layer"-type. The carbohydrate, recognition domain (CRD) of this galectin is common to those of animal, galectins, except for substitution of one residue, Ala64, which, corresponds to Asn46 in human galectin 1. A five-residue insertion in ACG, at positions 42-46 involving Ser44 and Asn46 modified the architecture of, the sugar binding site that contributes sialic acid specificity., Furthermore, it was found that the binding of a sulfate ion near the CRD, in the ligand-free form led to a change in the conformation of the loop, region caused by main-chain cis/trans transition between Ser44 and Pro45.
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<StructureSection load='1ww7' size='340' side='right'caption='[[1ww7]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ww7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrocybe_cylindracea Agrocybe cylindracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WW7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ww7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ww7 OCA], [https://pdbe.org/1ww7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ww7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ww7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ww7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ATLE_CYCAE ATLE_CYCAE] Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells. Binds N-acetylneuraminyl lactose (N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucose) (PubMed:16051274).<ref>PMID:12757412</ref> <ref>PMID:16051274</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ww/1ww7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ww7 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1WW7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Agrocybe_cylindracea Agrocybe cylindracea] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WW7 OCA].
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*[[Galectin 3D structures|Galectin 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural basis of a fungal galectin from Agrocybe cylindracea for recognizing sialoconjugate., Ban M, Yoon HJ, Demirkan E, Utsumi S, Mikami B, Yagi F, J Mol Biol. 2005 Aug 26;351(4):695-706. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16051274 16051274]
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__TOC__
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</StructureSection>
[[Category: Agrocybe cylindracea]]
[[Category: Agrocybe cylindracea]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Ban, M.]]
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[[Category: Ban M]]
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[[Category: Demirkan, E.]]
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[[Category: Demirkan E]]
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[[Category: Mikami, B.]]
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[[Category: Mikami B]]
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[[Category: Utsumi, S.]]
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[[Category: Utsumi S]]
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[[Category: Yagi, F.]]
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[[Category: Yagi F]]
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[[Category: Yoon, H.J.]]
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[[Category: Yoon HJ]]
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[[Category: SO4]]
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[[Category: agrocybe cylindracea galectin]]
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[[Category: carbohydrate recognition domain]]
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[[Category: fungal galectin]]
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[[Category: sulfate ion]]
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[[Category: x-ray crystallographic analysis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:42:50 2007''
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Current revision

Agrocybe cylindracea galectin (Ligand-free)

PDB ID 1ww7

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