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1xwo

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[[Image:1xwo.gif|left|200px]]
 
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{{Structure
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==crystal structrue of goose delta crystallin==
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|PDB= 1xwo |SIZE=350|CAPTION= <scene name='initialview01'>1xwo</scene>, resolution 2.8&Aring;
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<StructureSection load='1xwo' size='340' side='right'caption='[[1xwo]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1xwo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anser_anser_anser Anser anser anser]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XWO FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xwo OCA], [https://pdbe.org/1xwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xwo RCSB], [https://www.ebi.ac.uk/pdbsum/1xwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xwo ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xwo OCA], [http://www.ebi.ac.uk/pdbsum/1xwo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xwo RCSB]</span>
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[https://www.uniprot.org/uniprot/ARLY_ANSAN ARLY_ANSAN] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xw/1xwo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xwo ConSurf].
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<div style="clear:both"></div>
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'''crystal structrue of goose delta crystallin'''
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==See Also==
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*[[Crystallin 3D structures|Crystallin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Delta-crystallin is a soluble structural protein in avian eye lenses that confers special refractive properties. In the presence of GdmCl (guanidinium chloride), tetrameric delta-crystallin undergoes dissociation via a dimeric state to a monomeric molten globule intermediate state. The latter are denatured at higher GdmCl concentrations in a multi-state manner. In the present study, the X-ray structure of goose delta-crystallin was determined to 2.8 A (1 A=0.1 nm). In this structure the first 25 N-terminal residues interact with a hydrophobic cavity in a neighbouring molecule, stabilizing the quaternary structure of this protein. When these 25 residues were deleted this did not produce any gross structural changes, as judged by CD analysis, but slightly altered tryptophan fluorescence and ANS (8-anilino-1-naphthalenesulphonic acid) spectra. The dimeric form was significantly identified as judged by sedimentation velocity and nondenaturing gradient gel electrophoresis. This mutant had increased sensitivity to temperature denaturation and GdmCl concentrations of 0.3-1.0 M. This protein was destabilized about 3.3 kcal/mol (1 kcal=4.184 kJ) due to N-terminal truncation. After incubation at 37 degrees C N-terminal truncated proteins were prone to aggregation, suggesting the presence of the unstable dimeric conformation. An important role for the N-terminus in dimer assembly of goose delta-crystallin is proposed.
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==About this Structure==
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1XWO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Anser_anser_anser Anser anser anser]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XWO OCA].
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==Reference==
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The effect of N-terminal truncation on double-dimer assembly of goose delta-crystallin., Lee HJ, Lai YH, Wu SY, Chen YH, Biochem J. 2005 Dec 15;392(Pt 3):545-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16101585 16101585]
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[[Category: Anser anser anser]]
[[Category: Anser anser anser]]
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[[Category: Argininosuccinate lyase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Chang GG]]
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[[Category: Chang, G G.]]
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[[Category: Lai YH]]
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[[Category: Lai, Y H.]]
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[[Category: Lee HJ]]
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[[Category: Lee, H J.]]
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[[Category: Wu SY]]
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[[Category: Wu, S Y.]]
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[[Category: alpha helix bundle]]
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[[Category: double dimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:55:38 2008''
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Current revision

crystal structrue of goose delta crystallin

PDB ID 1xwo

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