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1yz7

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[[Image:1yz7.gif|left|200px]]
 
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==Crystal structure of a C-terminal segment of the alpha subunit of aIF2 from Pyrococcus abyssi==
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The line below this paragraph, containing "STRUCTURE_1yz7", creates the "Structure Box" on the page.
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<StructureSection load='1yz7' size='340' side='right'caption='[[1yz7]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1yz7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YZ7 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.26&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yz7 OCA], [https://pdbe.org/1yz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yz7 RCSB], [https://www.ebi.ac.uk/pdbsum/1yz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yz7 ProSAT]</span></td></tr>
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{{STRUCTURE_1yz7| PDB=1yz7 | SCENE= }}
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</table>
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== Function ==
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'''Crystal structure of a C-terminal segment of the alpha subunit of aIF2 from Pyrococcus abyssi'''
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[https://www.uniprot.org/uniprot/IF2A_PYRAB IF2A_PYRAB] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Eukaryotic and archaeal initiation factor 2 (e- and aIF2, respectively) are heterotrimeric proteins (alphabetagamma) supplying the small subunit of the ribosome with methionylated initiator tRNA. The gamma subunit forms the core of the heterotrimer. It resembles elongation factor EF1-A and ensures interaction with Met-tRNA(i)(Met). In the presence of the alpha subunit, which is composed of three domains, the gamma subunit expresses full tRNA binding capacity. This study reports the crystallographic structure of the intact aIF2alpha subunit from the archaeon Pyrococcus abyssi and that of a derived C-terminal fragment containing domains 2 and 3. The obtained structures are compared with those of N-terminal domains 1 and 2 of yeast and human eIF2alpha and with the recently determined NMR structure of human eIF2alpha. We show that the three-domain organization in the alpha subunit is conserved in archaea and eukarya. Domains 1 and 2 form a rigid body linked to a mobile third domain. Sequence comparisons establish that the most conserved regions in the aIF2alpha polypeptide lie at opposite sides of the protein, within domain 1 and domain 3, respectively. These two domains are known to exhibit RNA binding capacities. We propose that domain 3, which is known to glue the alpha subunit onto the gamma subunit, participates in Met-tRNA(i)(Met) binding while domain 1 recognizes either rRNA or mRNA on the ribosome. Thereby, the observed structural mobility within the e- and aIF2alpha molecules would be an integral part of the biological function of this subunit in the heterotrimeric e- and aIF2alphabetagamma factors.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yz/1yz7_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1YZ7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZ7 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yz7 ConSurf].
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Structure-function relationships of the intact aIF2alpha subunit from the archaeon Pyrococcus abyssi., Yatime L, Schmitt E, Blanquet S, Mechulam Y, Biochemistry. 2005 Jun 21;44(24):8749-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15952781 15952781]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pyrococcus abyssi]]
[[Category: Pyrococcus abyssi]]
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[[Category: Single protein]]
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[[Category: Blanquet S]]
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[[Category: Blanquet, S.]]
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[[Category: Mechulam Y]]
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[[Category: Mechulam, Y.]]
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[[Category: Schmitt E]]
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[[Category: Schmitt, E.]]
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[[Category: Yatime L]]
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[[Category: Yatime, L.]]
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[[Category: Alpha-beta domain]]
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[[Category: Helical domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:59:29 2008''
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Current revision

Crystal structure of a C-terminal segment of the alpha subunit of aIF2 from Pyrococcus abyssi

PDB ID 1yz7

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