1zkj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:39, 13 March 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1zkj.gif|left|200px]]
 
-
{{Structure
+
==Structural Basis for the Extended Substrate Spectrum of CMY-10, a Plasmid-Encoded Class C beta-lactamase==
-
|PDB= 1zkj |SIZE=350|CAPTION= <scene name='initialview01'>1zkj</scene>, resolution 1.55&Aring;
+
<StructureSection load='1zkj' size='340' side='right'caption='[[1zkj]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
<table><tr><td colspan='2'>[[1zkj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZKJ FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zkj OCA], [https://pdbe.org/1zkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zkj RCSB], [https://www.ebi.ac.uk/pdbsum/1zkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zkj ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zkj OCA], [http://www.ebi.ac.uk/pdbsum/1zkj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zkj RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/Q99QC1_KLEAE Q99QC1_KLEAE] This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.[ARBA:ARBA00003808]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zk/1zkj_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zkj ConSurf].
 +
<div style="clear:both"></div>
-
'''Structural Basis for the Extended Substrate Spectrum of CMY-10, a Plasmid-Encoded Class C beta-lactamase'''
+
==See Also==
-
 
+
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The emergence and dissemination of extended-spectrum (ES) beta-lactamases induce therapeutic failure and a lack of eradication of clinical isolates even by third-generation beta-lactam antibiotics like ceftazidime. CMY-10 is a plasmid-encoded class C beta-lactamase with a wide spectrum of substrates. Unlike the well-studied class C ES beta-lactamase from Enterobacter cloacae GC1, the Omega-loop does not affect the active site conformation and the catalytic activity of CMY-10. Instead, a three-amino-acid deletion in the R2-loop appears to be responsible for the ES activity of CMY-10. According to the crystal structure solved at 1.55 A resolution, the deletion significantly widens the R2 active site, which accommodates the R2 side-chains of beta-lactam antibiotics. This observation led us to demonstrate the hydrolysing activity of CMY-10 towards imipenem with a long R2 substituent. The forced mutational analyses of P99 beta-lactamase reveal that the introduction of deletion mutations into the R2-loop is able to extend the substrate spectrum of class C non-ES beta-lactamases, which is compatible with the isolation of natural class C ES enzymes harbouring deletion mutations in the R2-loop. Consequently, the opening of the R2 active site by the deletion of some residues in the R2-loop can be considered as an operative molecular strategy of class C beta-lactamases to extend their substrate spectrum.
+
[[Category: Klebsiella aerogenes]]
-
 
+
[[Category: Large Structures]]
-
==About this Structure==
+
[[Category: An YJ]]
-
1ZKJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacter_aerogenes Enterobacter aerogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZKJ OCA].
+
[[Category: Cha SS]]
-
 
+
[[Category: Jung HI]]
-
==Reference==
+
[[Category: Lee SH]]
-
Structural basis for the extended substrate spectrum of CMY-10, a plasmid-encoded class C beta-lactamase., Kim JY, Jung HI, An YJ, Lee JH, Kim SJ, Jeong SH, Lee KJ, Suh PG, Lee HS, Lee SH, Cha SS, Mol Microbiol. 2006 May;60(4):907-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16677302 16677302]
+
-
[[Category: Beta-lactamase]]
+
-
[[Category: Enterobacter aerogenes]]
+
-
[[Category: Single protein]]
+
-
[[Category: An, Y J.]]
+
-
[[Category: Cha, S S.]]
+
-
[[Category: Jung, H I.]]
+
-
[[Category: Lee, S H.]]
+
-
[[Category: class c]]
+
-
[[Category: cmy-10]]
+
-
[[Category: extended spectrum beta-lactamase]]
+
-
[[Category: plasmid]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:37:56 2008''
+

Current revision

Structural Basis for the Extended Substrate Spectrum of CMY-10, a Plasmid-Encoded Class C beta-lactamase

PDB ID 1zkj

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zkj"
Personal tools