2as9

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[[Image:2as9.gif|left|200px]]
 
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==Functional and structural characterization of Spl proteases from staphylococcus aureus==
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The line below this paragraph, containing "STRUCTURE_2as9", creates the "Structure Box" on the page.
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<StructureSection load='2as9' size='340' side='right'caption='[[2as9]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2as9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AS9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2as9| PDB=2as9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2as9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2as9 OCA], [https://pdbe.org/2as9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2as9 RCSB], [https://www.ebi.ac.uk/pdbsum/2as9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2as9 ProSAT]</span></td></tr>
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</table>
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'''Functional and structural characterization of Spl proteases from staphylococcus aureus'''
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== Function ==
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[https://www.uniprot.org/uniprot/SPLC_STAA8 SPLC_STAA8]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Staphylococcus aureus is the major cause of nosocomial infections world-wide, with increasing prevalence of community-acquired diseases. The recent dramatic increase in multi-antibiotic resistance, including resistance to the last-resort drug, vancomycin, together with the lack of an effective vaccine highlight the need for better understanding of S.aureus pathogenicity. Comparative analysis of available bacterial genomes allows for the identification of previously uncharacterized S.aureus genes with potential roles in pathogenicity. A good example is a cluster of six serine protease-like (spl) genes encompassed in one operon, which encode for putative proteases with similarity to staphylococcal glutamylendopeptidase (V8 protease). Here, we describe an efficient expression system for the production of recombinant SplB and SplC proteases in Escherichia coli, together with structural and functional characterization of the purified enzymes. A unique mechanism of cytoplasm protection against activity of misdirected SplB was uncovered. Apparently, the co-translated signal peptide maintains protease latency until it is cleaved by the signal peptidase during protein secretion. Furthermore, the crystal structure of the SplC protease revealed a fold resembling that of the V8 protease and epidermolytic toxins. Arrangement of the active site cleft and substrate-binding pocket of SplC explains the mechanism of enzyme latency and suggests that some Spl proteases possess restricted substrate specificity similar to that of the V8 protease and epidermolytic toxins.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/as/2as9_consurf.spt"</scriptWhenChecked>
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2AS9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AS9 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Functional and structural characterization of Spl proteases from Staphylococcus aureus., Popowicz GM, Dubin G, Stec-Niemczyk J, Czarny A, Dubin A, Potempa J, Holak TA, J Mol Biol. 2006 Apr 21;358(1):270-9. Epub 2006 Feb 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16516230 16516230]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2as9 ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Staphylococcus aureus]]
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__TOC__
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[[Category: Czarny, A.]]
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</StructureSection>
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[[Category: Dubin, A.]]
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[[Category: Large Structures]]
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[[Category: Dubin, G.]]
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[[Category: Holak, T A.]]
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[[Category: Popowicz, G M.]]
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[[Category: Potempa, J.]]
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[[Category: Stec-Niemczyk, J.]]
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[[Category: Serine protease]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Trypsin-like fold]]
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[[Category: Czarny A]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:25:05 2008''
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[[Category: Dubin A]]
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[[Category: Dubin G]]
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[[Category: Holak TA]]
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[[Category: Popowicz GM]]
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[[Category: Potempa J]]
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[[Category: Stec-Niemczyk J]]

Current revision

Functional and structural characterization of Spl proteases from staphylococcus aureus

PDB ID 2as9

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