2bd0

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[[Image:2bd0.gif|left|200px]]
 
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==Chlorobium tepidum Sepiapterin Reductase complexed with NADP and Sepiapterin==
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The line below this paragraph, containing "STRUCTURE_2bd0", creates the "Structure Box" on the page.
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<StructureSection load='2bd0' size='340' side='right'caption='[[2bd0]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2bd0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlorobaculum_tepidum_TLS Chlorobaculum tepidum TLS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BD0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BIO:BIOPTERIN'>BIO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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{{STRUCTURE_2bd0| PDB=2bd0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bd0 OCA], [https://pdbe.org/2bd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bd0 RCSB], [https://www.ebi.ac.uk/pdbsum/2bd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bd0 ProSAT]</span></td></tr>
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</table>
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'''Chlorobium tepidum Sepiapterin Reductase complexed with NADP and Sepiapterin'''
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== Function ==
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[https://www.uniprot.org/uniprot/SPRE_CHLTE SPRE_CHLTE] Catalyzes the final reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.<ref>PMID:10333495</ref> <ref>PMID:15621425</ref> <ref>PMID:18542834</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Sepiapterin reductase (SR) is involved in the last step of tetrahydrobiopterin (BH(4)) biosynthesis by reducing the di-keto group of 6-pyruvoyl tetrahydropterin. Chlorobium tepidum SR (cSR) generates a distinct BH(4) product, L-threo-BH(4) (6R-(1'S,2'S)-5,6,7,8-BH(4)), whereas animal enzymes produce L-erythro-BH(4) (6R-(1'R,2'S)-5,6,7,8-BH(4)) although it has high amino acid sequence similarities to the other animal enzymes. To elucidate the structural basis for the different reaction stereospecificities, we have determined the three-dimensional structures of cSR alone and complexed with NADP and sepiapterin at 2.1 and 1.7 A resolution, respectively. The overall folding of the cSR, the binding site for the cofactor NADP(H), and the positions of active site residues were quite similar to the mouse and the human SR. However, significant differences were found in the substrate binding region of the cSR. In comparison to the mouse SR complex, the sepiapterin in the cSR is rotated about 180 degrees around the active site and bound between two aromatic side chains of Trp-196 and Phe-99 so that its pterin ring is shifted to the opposite side, but its side chain position is not changed. The swiveled sepiapterin binding results in the conversion of the side chain configuration, exposing the opposite face for hydride transfer from NADPH. The different sepiapterin binding mode within the conserved catalytic architecture presents a novel strategy of switching the reaction stereospecificities in the same protein fold.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bd/2bd0_consurf.spt"</scriptWhenChecked>
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2BD0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlorobaculum_tepidum Chlorobaculum tepidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BD0 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of Chlorobium tepidum sepiapterin reductase complex reveals the novel substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin., Supangat S, Seo KH, Choi YK, Park YS, Son D, Han CD, Lee KH, J Biol Chem. 2006 Jan 27;281(4):2249-56. Epub 2005 Nov 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16308317 16308317]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bd0 ConSurf].
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[[Category: Chlorobaculum tepidum]]
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<div style="clear:both"></div>
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[[Category: Sepiapterin reductase]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Choi, Y K.]]
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__TOC__
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[[Category: Lee, K H.]]
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</StructureSection>
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[[Category: Park, Y S.]]
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[[Category: Chlorobaculum tepidum TLS]]
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[[Category: Seo, K H.]]
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[[Category: Large Structures]]
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[[Category: Supangat, S.]]
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[[Category: Choi YK]]
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[[Category: Chlorobium tepidum]]
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[[Category: Lee KH]]
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[[Category: Sepiapterin reductase]]
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[[Category: Park YS]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:07:41 2008''
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[[Category: Seo KH]]
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[[Category: Supangat S]]

Current revision

Chlorobium tepidum Sepiapterin Reductase complexed with NADP and Sepiapterin

PDB ID 2bd0

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