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2cxc

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[[Image:2cxc.gif|left|200px]]
 
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{{Structure
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==Crystal structure of archaeal transcription termination factor NusA==
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|PDB= 2cxc |SIZE=350|CAPTION= <scene name='initialview01'>2cxc</scene>, resolution 2.00&Aring;
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<StructureSection load='2cxc' size='340' side='right'caption='[[2cxc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2cxc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix_K1 Aeropyrum pernix K1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CXC FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxc OCA], [https://pdbe.org/2cxc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cxc RCSB], [https://www.ebi.ac.uk/pdbsum/2cxc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cxc ProSAT], [https://www.topsan.org/Proteins/RSGI/2cxc TOPSAN]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxc OCA], [http://www.ebi.ac.uk/pdbsum/2cxc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cxc RCSB]</span>
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[https://www.uniprot.org/uniprot/Q9YAU4_AERPE Q9YAU4_AERPE]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Crystal structure of archaeal transcription termination factor NusA'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/2cxc_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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The transcription factor NusA functions in transcriptional regulation involving termination in bacteria. A NusA homolog consisting of only the two KH domains is widely conserved in archaea, but its function remains unknown. We have found that Aeropyrum pernix NusA strongly binds to a certain CU-rich sequence near a termination signal. Our crystal structure of A. pernix NusA revealed that its spatial arrangement is quite similar to that of the KH domains of bacterial NusA. Thus, we consider archaeal NusA to have retained some functions of bacterial NusA, including the ssRNA-binding ability. Remarkable structural differences between archaeal and bacterial NusA exist at the interface with RNAP, in connection with the different NusA-binding sites around the termination signals. Transcriptional termination in archaea could differ from all of the known bacterial and eukaryal mechanisms, in terms of the combination of a bacterial factor and a eukaryal-type RNAP.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cxc ConSurf].
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2CXC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXC OCA].
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<div style="clear:both"></div>
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure and RNA-binding analysis of the archaeal transcription factor NusA., Shibata R, Bessho Y, Shinkai A, Nishimoto M, Fusatomi E, Terada T, Shirouzu M, Yokoyama S, Biochem Biophys Res Commun. 2007 Mar 30;355(1):122-8. Epub 2007 Jan 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17288993 17288993]
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[[Category: Aeropyrum pernix K1]]
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[[Category: Aeropyrum pernix]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Bessho Y]]
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[[Category: Bessho, Y.]]
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[[Category: Shibata R]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Shirouzu M]]
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[[Category: Shibata, R.]]
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[[Category: Yokoyama S]]
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[[Category: Shirouzu, M.]]
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[[Category: Yokoyama, S.]]
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[[Category: archaeal nusa]]
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[[Category: kh domain]]
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[[Category: national project on protein structural and functional analyse]]
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[[Category: nppsfa]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rna binding protein]]
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[[Category: rsgi]]
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[[Category: structural genomic]]
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[[Category: transcription termination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:27:35 2008''
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Current revision

Crystal structure of archaeal transcription termination factor NusA

PDB ID 2cxc

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