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2czr

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[[Image:2czr.gif|left|200px]]
 
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{{Structure
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==Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA==
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|PDB= 2czr |SIZE=350|CAPTION= <scene name='initialview01'>2czr</scene>, resolution 2.30&Aring;
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<StructureSection load='2czr' size='340' side='right'caption='[[2czr]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[2czr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis_KOD1 Thermococcus kodakarensis KOD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CZR FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2czr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2czr OCA], [https://pdbe.org/2czr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2czr RCSB], [https://www.ebi.ac.uk/pdbsum/2czr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2czr ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2czr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2czr OCA], [http://www.ebi.ac.uk/pdbsum/2czr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2czr RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q9V2W6_THEKO Q9V2W6_THEKO]
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__TOC__
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'''Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA'''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermococcus kodakarensis KOD1]]
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==Overview==
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[[Category: Inoue T]]
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TATA-binding protein (TBP)-interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1 (Tk-TIP26) is a possible transcription regulatory protein in Thermococcales. Here, we report the crystal structure of Tk-TIP26 determined at 2.3 A resolution with multiple-wavelength anomalous dispersion (MAD) method. The overall structure of Tk-TIP26 consists of two domains. The N-terminal domain forms an alpha/beta structure, in which three alpha-helices enclose the central beta-sheet. The topology of this domain is similar to that of holliday junction resolvase Hjc from Pyrococcus furiosus. The C-terminal domain comprises three alpha-helices, six beta-strands, and two 3(10)-helices. In the dimer structure of Tk-TIP26, two molecules are related with the crystallographic twofold axis, and these molecules rigidly interact with each other via hydrogen bonds. The complex of Tk-TIP26/Tk-TBP is isolated and analyzed by SDS-PAGE and gel filtration column chromatography, resulting in a stoichiometric ratio of the interaction between Tk-TIP26 and Tk-TBP of 4:2, i.e., two dimer molecules of Tk-TIP26 formed a complex with one dimeric TBP. The electrostatic surfaces of Tk-TIP26 and TBP from Pyrocuccus woesei (PwTBP) allowed us to build a model of the Tk-TIP26/TBP complex, and to propose the inhibition mechanism where two dimer molecules of Tk-TIP26 bind to a dimeric TBP, preventing its binding to TATA-DNA.
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[[Category: Kai Y]]
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[[Category: Kanaya S]]
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==About this Structure==
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[[Category: Matsuda T]]
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2CZR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_kodakarensis Thermococcus kodakarensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZR OCA].
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[[Category: Matsumura H]]
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[[Category: Morikawa M]]
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==Reference==
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[[Category: Yamamoto T]]
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Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA., Yamamoto T, Matsuda T, Inoue T, Matsumura H, Morikawa M, Kanaya S, Kai Y, Protein Sci. 2006 Jan;15(1):152-61. Epub 2005 Dec 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16322571 16322571]
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[[Category: Single protein]]
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[[Category: Thermococcus kodakarensis]]
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[[Category: Inoue, T.]]
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[[Category: Kai, Y.]]
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[[Category: Kanaya, S.]]
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[[Category: Matsuda, T.]]
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[[Category: Matsumura, H.]]
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[[Category: Morikawa, M.]]
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[[Category: Yamamoto, T.]]
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[[Category: hyperthermophilic archaeon]]
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[[Category: tata-binding protein (tbp)]]
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[[Category: tbp-interacting protein (tip)]]
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[[Category: zn-finger motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:28:24 2008''
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Current revision

Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA

PDB ID 2czr

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