2dpy

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[[Image:2dpy.jpg|left|200px]]<br /><applet load="2dpy" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2dpy, resolution 2.40&Aring;" />
 
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'''Crystal structure of the flagellar type III ATPase FliI'''<br />
 
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==Overview==
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==Crystal structure of the flagellar type III ATPase FliI==
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Construction of the bacterial flagellum in the cell exterior proceeds at its distal end by highly ordered self-assembly of many different component proteins, which are selectively exported through the central channel of the growing flagellum by the flagellar type III export apparatus. FliI is the ATPase of the export apparatus that drives the export process. Here we report the 2.4 A resolution crystal structure of FliI in the ADP-bound form. FliI consists of three domains, and the whole structure shows extensive similarities to the alpha and beta subunits of F0F1-ATPsynthase, a rotary motor that drives the chemical reaction of ATP synthesis. A hexamer model of FliI has been constructed based on the F1-ATPase structure composed of the alpha3beta3gamma subunits. Although the regions that differ in conformation between FliI and the F1-alpha/beta subunits are all located on the outer surface of the hexamer ring, the main chain structures at the subunit interface and those surrounding the central channel of the ring are well conserved. These results imply an evolutionary relation between the flagellum and F0F1-ATPsynthase and a similarity in the mechanism between FliI and F1-ATPase despite the apparently different functions of these proteins.
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<StructureSection load='2dpy' size='340' side='right'caption='[[2dpy]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2dpy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DPY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dpy OCA], [https://pdbe.org/2dpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dpy RCSB], [https://www.ebi.ac.uk/pdbsum/2dpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dpy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FLII_SALTY FLII_SALTY] Probable catalytic subunit of a protein translocase for flagellum-specific export, or a proton translocase involved in local circuits at the flagellum. May be involved in a specialized protein export pathway that proceeds without signal peptide cleavage.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/2dpy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dpy ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2DPY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DPY OCA].
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*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural similarity between the flagellar type III ATPase FliI and F1-ATPase subunits., Imada K, Minamino T, Tahara A, Namba K, Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):485-90. Epub 2007 Jan 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17202259 17202259]
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[[Category: Large Structures]]
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[[Category: H(+)-transporting two-sector ATPase]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Salmonella typhimurium]]
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[[Category: Imada K]]
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[[Category: Single protein]]
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[[Category: Minamino T]]
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[[Category: Imada, K.]]
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[[Category: Namba K]]
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[[Category: Minamino, T.]]
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[[Category: Namba, K.]]
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[[Category: ADP]]
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[[Category: alpha-beta structure]]
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[[Category: beta barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:01:22 2008''
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Current revision

Crystal structure of the flagellar type III ATPase FliI

PDB ID 2dpy

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