2dzn

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(New page: 200px<br /><applet load="2dzn" size="450" color="white" frame="true" align="right" spinBox="true" caption="2dzn, resolution 2.20&Aring;" /> '''Crystal structure an...)
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[[Image:2dzn.jpg|left|200px]]<br /><applet load="2dzn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2dzn, resolution 2.20&Aring;" />
 
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'''Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3'''<br />
 
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==Overview==
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==Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3==
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The 26S proteasome-dependent protein degradation is an evolutionarily, conserved process. The mammalian oncoprotein gankyrin, which associates, with S6 of the proteasome, facilitates the degradation of pRb, and thus, possibly acts as a bridging factor between the proteasome and its, substrates. However, the mechanism of the proteasome-dependent protein, degradation in yeast is poorly understood. Here, we report the tertiary, structure of the complex between Nas6 and a C-terminal domain of Rpt3, which are the yeast orthologues of gankyrin and S6, respectively. The, concave region of Nas6 bound to the alpha-helical domain of Rpt3. The, stable interaction between Nas6 and Rpt3 was mediated by intermolecular, interactions composed of complementary charged patches. The recognition of, Rpt3 by Nas6 in the crystal suggests that Nas6 is indeed a subunit of the, 26S proteasome. These results provide a structural basis for the, association between Nas6 and the heterohexameric ATPase ring of the, proteasome through Rpt3.
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<StructureSection load='2dzn' size='340' side='right'caption='[[2dzn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2dzn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DZN FirstGlance]. <br>
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2DZN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DZN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dzn OCA], [https://pdbe.org/2dzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dzn RCSB], [https://www.ebi.ac.uk/pdbsum/2dzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dzn ProSAT], [https://www.topsan.org/Proteins/RSGI/2dzn TOPSAN]</span></td></tr>
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==Reference==
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</table>
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Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome., Nakamura Y, Umehara T, Tanaka A, Horikoshi M, Padmanabhan B, Yokoyama S, Biochem Biophys Res Commun. 2007 Aug 3;359(3):503-9. Epub 2007 May 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17555716 17555716]
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== Function ==
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[[Category: Protein complex]]
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[https://www.uniprot.org/uniprot/PSD10_YEAST PSD10_YEAST] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.<ref>PMID:19412159</ref> <ref>PMID:19446322</ref> <ref>PMID:9714768</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dz/2dzn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dzn ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Padmanabhan, B.]]
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[[Category: Padmanabhan B]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Yokoyama S]]
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[[Category: Yokoyama, S.]]
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[[Category: a-helical domain]]
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[[Category: ankyrin repeats]]
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[[Category: national project on protein structural and functional analyses]]
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[[Category: nppsfa]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:52:31 2007''
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Current revision

Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3

PDB ID 2dzn

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