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2eb7

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[[Image:2eb7.jpg|left|200px]]<br /><applet load="2eb7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2eb7, resolution 1.80&Aring;" />
 
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'''Crystal structure of the hypothetical regulator from Sulfolobus tokodaii 7'''<br />
 
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==Overview==
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==Crystal structure of the hypothetical regulator from Sulfolobus tokodaii 7==
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The emergence of bacterial resistance to multiple drugs poses a serious and growing health concern. Understanding and deciphering the mechanisms of these multiple drug resistance regulatory proteins through structural or biochemical means is an important endeavor. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 in two different crystal forms, at 1.80 and 2.0A, respectively. The overall structure of the ST1710 dimer shares the topology of the MarR family of proteins, with each subunit containing a winged helix-turn-helix DNA-binding motif. We also show the protein-DNA interactions by biochemical methods. Our molecular modeling analysis suggested that Asp88 and Arg90 are the key residues in ST1710 involved in the protein-DNA interactions.
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<StructureSection load='2eb7' size='340' side='right'caption='[[2eb7]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2eb7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii_str._7 Sulfurisphaera tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EB7 FirstGlance]. <br>
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2EB7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EB7 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eb7 OCA], [https://pdbe.org/2eb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eb7 RCSB], [https://www.ebi.ac.uk/pdbsum/2eb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eb7 ProSAT], [https://www.topsan.org/Proteins/RSGI/2eb7 TOPSAN]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7., Kumarevel T, Tanaka T, Nishio M, Gopinath SC, Takio K, Shinkai A, Kumar PK, Yokoyama S, J Struct Biol. 2008 Jan;161(1):9-17. Epub 2007 Sep 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17933554 17933554]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q96ZY1_SULTO Q96ZY1_SULTO]
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[[Category: Sulfolobus tokodaii]]
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== Evolutionary Conservation ==
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[[Category: Kumarevel, T S.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Nishio, M.]]
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Check<jmol>
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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<jmolCheckbox>
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[[Category: Shinkai, A.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/2eb7_consurf.spt"</scriptWhenChecked>
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[[Category: Yokoyama, S.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: hypothetical regulator]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: mar family]]
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</jmolCheckbox>
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[[Category: national project on protein structural and functional analyses]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eb7 ConSurf].
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[[Category: nppsfa]]
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<div style="clear:both"></div>
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[[Category: riken structural genomics/proteomics initiative]]
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__TOC__
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[[Category: rsgi]]
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</StructureSection>
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[[Category: st1710]]
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[[Category: Large Structures]]
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[[Category: structural genomics]]
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[[Category: Sulfurisphaera tokodaii str. 7]]
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[[Category: transcription regulator]]
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[[Category: Kumarevel TS]]
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[[Category: Nishio M]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:08:10 2008''
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[[Category: Shinkai A]]
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[[Category: Yokoyama S]]

Current revision

Crystal structure of the hypothetical regulator from Sulfolobus tokodaii 7

PDB ID 2eb7

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