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2ewt

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(New page: 200px<br /><applet load="2ewt" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ewt, resolution 1.81&Aring;" /> '''Crystal structure of...)
Current revision (13:50, 13 March 2024) (edit) (undo)
 
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[[Image:2ewt.gif|left|200px]]<br /><applet load="2ewt" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ewt, resolution 1.81&Aring;" />
 
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'''Crystal structure of the DNA-binding domain of BldD'''<br />
 
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==Overview==
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==Crystal structure of the DNA-binding domain of BldD==
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BldD is a central regulator of the developmental process in Streptomyces, coelicolor. The 1.8 angstroms resolution structure of the DNA-binding, domain of BldD (BldDN) reveals that BldDN forms a compact globular domain, composed of four helices (alpha1-alpha4) containing a helix-turn-helix, motif (alpha2-alpha3) resembling that of the DNA-binding domain of lambda, repressor. The BldDN/DNA complex model led us to design a series of, mutants, which revealed the important role of alpha3 and the 'turn' region, between alpha2 and alpha3 for DNA recognition. Based on the fact that BldD, occupies two operator sites of bldN and whiG and shows significant, disparity in the affinity toward the two operator sites when they are, disconnected, we propose a model of cooperative binding, which means that, the binding of one BldD dimer to the high affinity site facilitates that, of the second BldD dimer to the low affinity site. In addition, structural, and mutational investigation reveals that the Tyr62Cys mutation, found in, the first-identified bldD mutant, can destabilize BldD structure by, disrupting the hydrophobic core.
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<StructureSection load='2ewt' size='340' side='right'caption='[[2ewt]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2ewt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EWT FirstGlance]. <br>
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2EWT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWT OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ewt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ewt OCA], [https://pdbe.org/2ewt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ewt RCSB], [https://www.ebi.ac.uk/pdbsum/2ewt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ewt ProSAT]</span></td></tr>
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Crystal structure of the DNA-binding domain of BldD, a central regulator of aerial mycelium formation in Streptomyces coelicolor A3(2)., Kim IK, Lee CJ, Kim MK, Kim JM, Kim JH, Yim HS, Cha SS, Kang SO, Mol Microbiol. 2006 Jun;60(5):1179-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16689794 16689794]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Streptomyces coelicolor]]
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[https://www.uniprot.org/uniprot/Q7AKQ8_STRCO Q7AKQ8_STRCO]
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[[Category: Cha, S.S.]]
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== Evolutionary Conservation ==
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[[Category: Kang, S.O.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Kim, I.K.]]
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Check<jmol>
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[[Category: Kim, J.H.]]
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<jmolCheckbox>
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[[Category: Kim, J.M.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/2ewt_consurf.spt"</scriptWhenChecked>
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[[Category: Kim, M.K.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Lee, C.J.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Yim, H.S.]]
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</jmolCheckbox>
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[[Category: SO4]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ewt ConSurf].
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[[Category: the dna-binding domain of bldd]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:23:30 2008''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Cha SS]]
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[[Category: Kang SO]]
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[[Category: Kim IK]]
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[[Category: Kim JH]]
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[[Category: Kim JM]]
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[[Category: Kim MK]]
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[[Category: Lee CJ]]
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[[Category: Yim HS]]

Current revision

Crystal structure of the DNA-binding domain of BldD

PDB ID 2ewt

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