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2ful

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[[Image:2ful.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of the C-terminal Domain of S. cerevisiae eIF5==
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|PDB= 2ful |SIZE=350|CAPTION= <scene name='initialview01'>2ful</scene>, resolution 1.500&Aring;
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<StructureSection load='2ful' size='340' side='right'caption='[[2ful]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[2ful]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FUL FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ful FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ful OCA], [https://pdbe.org/2ful PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ful RCSB], [https://www.ebi.ac.uk/pdbsum/2ful PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ful ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ful FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ful OCA], [http://www.ebi.ac.uk/pdbsum/2ful PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ful RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/IF5_YEAST IF5_YEAST] Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). eIF-5 is essential for cell viability.<ref>PMID:12651896</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/2ful_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ful ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of the C-terminal Domain of S. cerevisiae eIF5'''
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==See Also==
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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== References ==
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==Overview==
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<references/>
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eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-terminal domain (CTD) of eIF5 functions as the structural core in the MFC assembly. Here we report the 1.5A crystal structure of eIF5-CTD, confirming that eIF5-CTD contains an atypical HEAT motif. In addition, analyzing the electrostatic potential and the distribution of conserved residues on the protein surface, we confirm and suggest some potential regions of interactions between eIF5-CTD and other eIFs. The structure of eIF5-CTD provides useful information in understanding the mechanism of the MFC assembly.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2FUL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FUL OCA].
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==Reference==
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Crystal structure of the C-terminal domain of S.cerevisiae eIF5., Wei Z, Xue Y, Xu H, Gong W, J Mol Biol. 2006 May 26;359(1):1-9. Epub 2006 Mar 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16616930 16616930]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Gong W]]
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[[Category: Gong, W.]]
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[[Category: Wei Z]]
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[[Category: Wei, Z.]]
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[[Category: Xu H]]
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[[Category: Xu, H.]]
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[[Category: Xue Y]]
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[[Category: Xue, Y.]]
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[[Category: atypical heat motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:07:53 2008''
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Current revision

Crystal Structure of the C-terminal Domain of S. cerevisiae eIF5

PDB ID 2ful

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