2ful

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2ful" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ful, resolution 1.500&Aring;" /> '''Crystal Structure o...)
Current revision (13:51, 13 March 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2ful.gif|left|200px]]<br /><applet load="2ful" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2ful, resolution 1.500&Aring;" />
 
-
'''Crystal Structure of the C-terminal Domain of S. cerevisiae eIF5'''<br />
 
-
==Overview==
+
==Crystal Structure of the C-terminal Domain of S. cerevisiae eIF5==
-
eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a, critical role in pre-initiation complex assembly and correct AUG selection, during eukaryotic translation initiation. eIF5 is involved in the, formation of the multifactor complex (MFC), an important intermediate of, the 43S pre-initiation complex. The C-terminal domain (CTD) of eIF5, functions as the structural core in the MFC assembly. Here we report the, 1.5A crystal structure of eIF5-CTD, confirming that eIF5-CTD contains an, atypical HEAT motif. In addition, analyzing the electrostatic potential, and the distribution of conserved residues on the protein surface, we, confirm and suggest some potential regions of interactions between, eIF5-CTD and other eIFs. The structure of eIF5-CTD provides useful, information in understanding the mechanism of the MFC assembly.
+
<StructureSection load='2ful' size='340' side='right'caption='[[2ful]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2ful]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FUL FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ful FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ful OCA], [https://pdbe.org/2ful PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ful RCSB], [https://www.ebi.ac.uk/pdbsum/2ful PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ful ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/IF5_YEAST IF5_YEAST] Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). eIF-5 is essential for cell viability.<ref>PMID:12651896</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/2ful_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ful ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
2FUL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FUL OCA].
+
*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
Crystal structure of the C-terminal domain of S.cerevisiae eIF5., Wei Z, Xue Y, Xu H, Gong W, J Mol Biol. 2006 May 26;359(1):1-9. Epub 2006 Mar 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16616930 16616930]
+
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Single protein]]
+
[[Category: Gong W]]
-
[[Category: Gong, W.]]
+
[[Category: Wei Z]]
-
[[Category: Wei, Z.]]
+
[[Category: Xu H]]
-
[[Category: Xu, H.]]
+
[[Category: Xue Y]]
-
[[Category: Xue, Y.]]
+
-
[[Category: SO4]]
+
-
[[Category: atypical heat motif]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:46:56 2007''
+

Current revision

Crystal Structure of the C-terminal Domain of S. cerevisiae eIF5

PDB ID 2ful

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ful"
Personal tools