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2gwo

From Proteopedia

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[[Image:2gwo.jpg|left|200px]]
 
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{{Structure
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==crystal structure of TMDP==
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|PDB= 2gwo |SIZE=350|CAPTION= <scene name='initialview01'>2gwo</scene>, resolution 2.4&Aring;
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<StructureSection load='2gwo' size='340' side='right'caption='[[2gwo]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2gwo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GWO FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE= DUSP13, TMDP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gwo OCA], [https://pdbe.org/2gwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gwo RCSB], [https://www.ebi.ac.uk/pdbsum/2gwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gwo ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DS13B_HUMAN DS13B_HUMAN] Dual specificity phosphatase that dephosphorylates MAPK8/JNK and MAPK14/p38, but not MAPK1/ERK2, in vitro. Exhibits intrinsic phosphatase activity towards both phospho-seryl/threonyl and -tyrosyl residues, with similar specific activities in vitro.<ref>PMID:21360282</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gw/2gwo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gwo ConSurf].
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<div style="clear:both"></div>
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'''crystal structure of TMDP'''
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==See Also==
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*[[Dual specificity phosphatase 3D structures|Dual specificity phosphatase 3D structures]]
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== References ==
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==Overview==
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<references/>
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The testis- and skeletal-muscle-specific dual-specificity phosphatase (TMDP) is a member of the dual-specificity phosphatase (DSP) subgroup of protein tyrosine phosphatases. TMDP has similar activities toward both tyrosine and threonine phosphorylated substrates, and is supposed to be involved in spermatogenesis. Here, we report the crystal structure of human TMDP at a resolution of 2.4 A. In spite of high sequence similarity with other DSPs, the crystal structure of TMDP shows distinct structural motifs and surface properties. In TMDP, the alpha1-beta1 loop, a substrate recognition motif is located further away from the active site loop in comparison to prototype DSP Vaccinia H1 related phophatase (VHR), which preferentially dephosphorylates tyrosine phosphorylated substrates and down-regulates MAP kinase signaling. Residues in the active site residues of TMDP are smaller in size and more hydrophobic than those of VHR. In addition, TMDP cannot be aligned with VHR in loop beta3-alpha4. These differences in the active site of TMDP result in a flat and wide pocket structure, allowing equal binding of phosphotyrosine and phosphothreonine substrates.
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__TOC__
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</StructureSection>
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==About this Structure==
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2GWO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWO OCA].
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==Reference==
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Crystal structure of human TMDP, a testis-specific dual specificity protein phosphatase: implications for substrate specificity., Kim SJ, Jeong DG, Yoon TS, Son JH, Cho SK, Ryu SE, Kim JH, Proteins. 2007 Jan 1;66(1):239-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17044055 17044055]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kim, J H.]]
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[[Category: Kim JH]]
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[[Category: Kim, S J.]]
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[[Category: Kim SJ]]
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[[Category: Ryu, S E.]]
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[[Category: Ryu SE]]
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[[Category: alpha/beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:10:03 2008''
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Current revision

crystal structure of TMDP

PDB ID 2gwo

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