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2pwt

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[[Image:2pwt.gif|left|200px]]<br /><applet load="2pwt" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2pwt, resolution 1.800&Aring;" />
 
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'''Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group'''<br />
 
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==Overview==
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==Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group==
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The crystal structure of the complex between oligonucleotide containing the bacterial ribosomal decoding site (A site) and the synthetic paromomycin analogue 1, which contains the gamma-amino-alpha-hydroxybutyryl (L-haba) group at position N1 of ring II (2-DOS ring), and an ether chain with an O-phenethylaminoethyl group at position C2'' of ring III, is reported. Interestingly, next to the paromomycin analogue 1 specifically bound to the A site, a second molecule of 1 with a different conformation is observed at the crystal packing interface which mimics the A-minor interaction between two bulged-out adenines from the A site and the codon-anticodon stem of the mRNA-tRNA complex. Improved antibacterial activity supports the conclusion that analogue 1 might affect protein synthesis on the ribosome in two different ways: 1) specific binding to the A site forces maintenance of the "on" state with two bulged out adenines, and 2) a new binding mode of 1 to an A-minor motif which stabilizes complex formation between the ribosome and the mRNA-tRNA complex regardless of whether the codon-anticodon stem is of the cognate or near-cognate type.
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<StructureSection load='2pwt' size='340' side='right'caption='[[2pwt]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2pwt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PWT FirstGlance]. <br>
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2PWT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=LHA:'>LHA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWT OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHA:DOUBLY+FUNCTIONALIZED+PAROMOMYCIN+PM-II-162'>LHA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pwt OCA], [https://pdbe.org/2pwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pwt RCSB], [https://www.ebi.ac.uk/pdbsum/2pwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pwt ProSAT]</span></td></tr>
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Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity., Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E, ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17722211 17722211]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Francois, B.]]
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</StructureSection>
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[[Category: Hanessian, S.]]
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[[Category: Large Structures]]
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[[Category: Kondo, J.]]
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[[Category: Francois B]]
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[[Category: Pachamuthu, K.]]
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[[Category: Hanessian S]]
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[[Category: Szychowski, J.]]
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[[Category: Kondo J]]
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[[Category: Westhof, E.]]
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[[Category: Pachamuthu K]]
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[[Category: LHA]]
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[[Category: Szychowski J]]
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[[Category: aminoglycoside; haba group; ribosomal decoding site; x-ray analysis; rna]]
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[[Category: Westhof E]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:33:46 2008''
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Current revision

Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group

PDB ID 2pwt

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