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2z8x

From Proteopedia

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[[Image:2z8x.gif|left|200px]]<br /><applet load="2z8x" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2z8x, resolution 1.48&Aring;" />
 
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'''Crystal structure of extracellular lipase from Pseudomonas sp. MIS38'''<br />
 
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==Overview==
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==Crystal structure of extracellular lipase from Pseudomonas sp. MIS38==
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The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.
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<StructureSection load='2z8x' size='340' side='right'caption='[[2z8x]], [[Resolution|resolution]] 1.48&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2z8x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._MIS38 Pseudomonas sp. MIS38]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z8X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.48&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8x OCA], [https://pdbe.org/2z8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z8x RCSB], [https://www.ebi.ac.uk/pdbsum/2z8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z8x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9RBY1_9PSED Q9RBY1_9PSED]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z8/2z8x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z8x ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2Z8X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._mis38 Pseudomonas sp. mis38] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8X OCA].
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*[[Lipase 3D Structures|Lipase 3D Structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation., Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S, FEBS Lett. 2007 Oct 30;581(26):5060-4. Epub 2007 Oct 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17923123 17923123]
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[[Category: Large Structures]]
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[[Category: Pseudomonas sp. mis38]]
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[[Category: Pseudomonas sp. MIS38]]
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[[Category: Single protein]]
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[[Category: Angkawidjaja C]]
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[[Category: Triacylglycerol lipase]]
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[[Category: Kanaya S]]
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[[Category: Angkawidjaja, C.]]
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[[Category: Koga Y]]
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[[Category: Kanaya, S.]]
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[[Category: Matsumura H]]
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[[Category: Koga, Y.]]
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[[Category: Takano K]]
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[[Category: Matsumura, H.]]
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[[Category: You DJ]]
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[[Category: Takano, K.]]
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[[Category: You, D J.]]
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[[Category: CA]]
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[[Category: ZN]]
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[[Category: beta roll]]
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[[Category: calcium binding protein]]
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[[Category: family i 3 lipase]]
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[[Category: hydrolase]]
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[[Category: rtx protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:00:50 2008''
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Current revision

Crystal structure of extracellular lipase from Pseudomonas sp. MIS38

PDB ID 2z8x

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