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3afk

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<StructureSection load='3afk' size='340' side='right'caption='[[3afk]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
<StructureSection load='3afk' size='340' side='right'caption='[[3afk]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3afk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrae Agrae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AFK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3afk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyclocybe_aegerita Cyclocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AFK FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=THR:THREONINE'>THR</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3m3c|3m3c]], [[3m3q|3m3q]], [[3m3e|3m3e]], [[3m3o|3m3o]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900084:Thomsen-Friedenreich+antigen'>PRD_900084</scene>, <scene name='pdbligand=THR:THREONINE'>THR</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AAL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5400 AGRAE])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3afk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3afk OCA], [https://pdbe.org/3afk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3afk RCSB], [https://www.ebi.ac.uk/pdbsum/3afk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3afk ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3afk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3afk OCA], [https://pdbe.org/3afk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3afk RCSB], [https://www.ebi.ac.uk/pdbsum/3afk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3afk ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/ATLE_AGRAE ATLE_AGRAE]] Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells.<ref>PMID:12757412</ref>
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[https://www.uniprot.org/uniprot/ATLE_CYCAE ATLE_CYCAE] Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells. Binds N-acetylneuraminyl lactose (N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucose) (PubMed:16051274).<ref>PMID:12757412</ref> <ref>PMID:16051274</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Thomsen-Friedenreich (TF) antigen, which plays an important role in the regulation of cancer cell proliferation, occurs in approximately 90% of all human cancers and precancerous conditions. Although TF antigen has been known for almost 80 yr as a pancarcinoma antigen, the recognition mechanism between TF antigen and target protein has not been structurally characterized. A number of studies indicated that TF disaccharide is a potential ligand of the galactoside-binding galectins. In this work, we identified the TF antigen as a potential ligand of the antitumor galectin AAL (Agrocybe aegerita lectin) through glycan array analysis and reported the crystal structure of AAL complexed with the TF antigen. The structure provides a first look at the recognition mode between AAL and TF antigen, which is unique in a conservative (Glu-water-Arg-water) structural motif-based hydrogen bond network. Structure-based mutagenesis analysis further revealed the residues responsible for recognition specificity and binding affinity. Crystal structures of AAL complexed with two other TF-containing glycans showed that the unique TF recognition mode is kept intact, which may be commonly adopted in some cancer-related galectins. The finding provided the new target and approach for the antitumor drug design and relative strategy based on the AAL-TF recognition mode as a prototype model.
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Structural insights into the recognition mechanism between an antitumor galectin AAL and the Thomsen-Friedenreich antigen.,Feng L, Sun H, Zhang Y, Li DF, Wang DC FASEB J. 2010 Oct;24(10):3861-8. Epub 2010 Jun 7. PMID:20530247<ref>PMID:20530247</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3afk" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Agrae]]
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[[Category: Cyclocybe aegerita]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Feng, L]]
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[[Category: Feng L]]
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[[Category: Li, D]]
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[[Category: Li D]]
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[[Category: Wang, D]]
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[[Category: Wang D]]
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[[Category: 3-galnac-alpha-o-thr]]
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[[Category: Aal]]
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[[Category: Apoptosis]]
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[[Category: Gal-beta-1]]
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[[Category: Galectin]]
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[[Category: Hydrolase]]
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[[Category: Lectin]]
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[[Category: Nuclease]]
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[[Category: Thomsen-friedenreich antigen]]
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Current revision

Crystal Structure of Agrocybe aegerita lectin AAL complexed with Thomsen-Friedenreich antigen

PDB ID 3afk

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