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3awd

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==Crystal structure of gox2181==
==Crystal structure of gox2181==
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<StructureSection load='3awd' size='340' side='right' caption='[[3awd]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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<StructureSection load='3awd' size='340' side='right'caption='[[3awd]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3awd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"acetobacter_capsulatum"_(sic)_shimwell_1936 "acetobacter capsulatum" (sic) shimwell 1936]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AWD FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3awd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gluconobacter_oxydans Gluconobacter oxydans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AWD FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GOX2181 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=442 "Acetobacter capsulatum" (sic) Shimwell 1936])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3awd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awd OCA], [http://pdbe.org/3awd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3awd RCSB], [http://www.ebi.ac.uk/pdbsum/3awd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3awd ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3awd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awd OCA], [https://pdbe.org/3awd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3awd RCSB], [https://www.ebi.ac.uk/pdbsum/3awd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3awd ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5FNX9_GLUOX Q5FNX9_GLUOX]
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Gluconobacter oxydans enable to oxidize sugars and polyols incompletely to corresponding materials with potential industrial applications, containing around 75 putative dehydrogenases. One of these putative dehydrogenases, Gox2181, was cloned and expressed in Escherichia coli BL21 (DE3), and its X-ray crystal structure was determined to a resolution of 1.8 A. Gox2181 formed a homo-tetramer in the crystal that was coincident with the apparent molecular mass determined in the solution. Gox2181 displayed alpha/beta-folding patterns, the conserved catalytic tetrad of Asn119-Ser147-Tyr162-Lys166, and the NAD-binding pocket, which aligned well with the 'classical' type of short-chain dehydrogenase/reductase (SDR) enzymes. Gox2181 was denoted SDR51C based on the SDR nomenclature system. The purified recombinant Gox2181 was demonstrated to be NAD(H)-dependent and active towards a wide range of substrates, including sugar alcohols, secondary alcohols, ketones, and ketoses. Among the substrates tested, Gox2181 displayed preference for secondary hydroxyl or carbonyl groups, showing low K(m) values with d-arabitol and butanedione.
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Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans.,Liu X, Yuan Z, Adam Yuan Y, Lin J, Wei D Biochem Biophys Res Commun. 2011 Nov 18;415(2):410-5. Epub 2011 Oct 21. PMID:22040731<ref>PMID:22040731</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3awd" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Yuan, Y Adam]]
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[[Category: Gluconobacter oxydans]]
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[[Category: Yuan, Z]]
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[[Category: Large Structures]]
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[[Category: Dehydrogenase]]
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[[Category: Adam Yuan Y]]
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[[Category: Oxidoreductase]]
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[[Category: Yuan Z]]

Current revision

Crystal structure of gox2181

PDB ID 3awd

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