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3b1x

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==Crystal structure of an S. thermophilus NFeoB E66A mutant bound to GMPPNP==
==Crystal structure of an S. thermophilus NFeoB E66A mutant bound to GMPPNP==
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<StructureSection load='3b1x' size='340' side='right' caption='[[3b1x]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
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<StructureSection load='3b1x' size='340' side='right'caption='[[3b1x]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3b1x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strt2 Strt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B1X FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3b1x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_thermophilus_LMG_18311 Streptococcus thermophilus LMG 18311]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B1X FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.61&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3lx5|3lx5]], [[3lx8|3lx8]], [[3ss8|3ss8]], [[3b1v|3b1v]], [[3b1w|3b1w]], [[3b1y|3b1y]], [[3b1z|3b1z]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FeoB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264199 STRT2])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b1x OCA], [https://pdbe.org/3b1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b1x RCSB], [https://www.ebi.ac.uk/pdbsum/3b1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b1x ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b1x OCA], [http://pdbe.org/3b1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b1x RCSB], [http://www.ebi.ac.uk/pdbsum/3b1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3b1x ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5M586_STRT2 Q5M586_STRT2]
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The acquisition of ferrous iron in prokaryotes is achieved by the G-protein-coupled membrane protein FeoB. This protein possesses a large C-terminal membrane-spanning domain preceded by two soluble cytoplasmic domains that are together termed 'NFeoB'. The first of these soluble domains is a GTPase domain (G-domain), which is then followed by an entirely alpha-helical domain. GTP hydrolysis by the G-domain is essential for iron uptake by FeoB, and various NFeoB mutant proteins from Streptococcus thermophilus have been constructed. These mutations investigate the role of conserved amino acids from the protein's critical Switch regions. Five crystal structures of these mutant proteins have been determined. The structures of E66A and E67A mutant proteins were solved in complex with nonhydrolyzable GTP analogues, the structures of T35A and E67A mutant proteins were solved in complex with GDP and finally the structure of the T35S mutant was crystallized without bound nucleotide. As an ensemble, the structures illustrate how small nucleotide-dependent rearrangements at the active site are converted into large rigid-body reorientations of the helical domain in response to GTP binding and hydrolysis. This provides the first evidence of nucleotide-dependent helical domain movement in NFeoB proteins, suggesting a mechanism by which the G-protein domain could structurally communicate with the membrane domain and mediate iron uptake.
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A suite of Switch I and Switch II mutant structures from the G-protein domain of FeoB.,Ash MR, Maher MJ, Guss JM, Jormakka M Acta Crystallogr D Biol Crystallogr. 2011 Nov;67(Pt 11):973-80. Epub 2011 Oct 19. PMID:22101824<ref>PMID:22101824</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3b1x" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Strt2]]
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[[Category: Large Structures]]
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[[Category: Ash, M R]]
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[[Category: Streptococcus thermophilus LMG 18311]]
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[[Category: Guss, J M]]
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[[Category: Ash MR]]
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[[Category: Jormakka, M]]
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[[Category: Guss JM]]
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[[Category: Maher, M J]]
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[[Category: Jormakka M]]
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[[Category: G protein]]
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[[Category: Maher MJ]]
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[[Category: Gtpase]]
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[[Category: Iron transport]]
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[[Category: Metal transport]]
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[[Category: Potassium]]
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[[Category: Transmembrane]]
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Current revision

Crystal structure of an S. thermophilus NFeoB E66A mutant bound to GMPPNP

PDB ID 3b1x

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