4fof

<StructureSection load='4fof' size='340' side='right' caption='[[4fof]], [[Resolution|resolution]] 2.42&Aring;' scene=''>

<table><tr><td colspan='2'>[[4fof]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Theeb Theeb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FOF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FOF FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=VRB:PHYCOVIOLOBILIN,+BLUE+LIGHT-ABSORBING+FORM'>VRB</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tll0569 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB]), HO1 and PcyA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fof OCA], [http://pdbe.org/4fof PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fof RCSB], [http://www.ebi.ac.uk/pdbsum/4fof PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fof ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

The phytochrome superfamily encompasses a diverse collection of photochromic photoreceptors in plants and microorganisms that employ a covalently linked bilin cradled in a cGMP-phosphodiesterase/adenylyl-cyclase/FhlA (GAF) domain to detect light. Whereas most interconvert between red- and far-red-light-absorbing states, cyanobacteria also express variants called cyanobacteriochromes (CBCRs) that modify bilin absorption to collectively perceive the entire visible spectrum. Here, we present two X-ray crystallographic structures of the GAF domain from the blue/green photochromic CBCR PixJ from Thermosynechococcus elongatus. These structures confirm the hypothesis that CBCRs variably manipulate the chromophore pi-conjugation system through isomerization and a second thioether linkage, in this case involving the bilin C10 carbon and Cys494 within a DXCF sequence characteristic of blue/green CBCRs. Biochemical studies support a mechanism for photoconversion whereby the second linkage ruptures on route to the green-light-absorbing state. Collectively, the TePixJ(GAF) models illustrate the remarkable structural and photochemical versatility among phytochromes and CBCRs in driving light perception.

 

== References ==

[[Category: Theeb]]

[[Category: Burgie, E S]]

[[Category: Gaf-domain]]

[[Category: Photoreceptor]]

[[Category: Phycoviolobilin]]

[[Category: Phytochrome]]

[[Category: Signaling protein]]