This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4lho

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:07, 13 March 2024) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal Structure of FG41Malonate Semialdehyde Decarboxylase inhibited by 3-bromopropiolate==
==Crystal Structure of FG41Malonate Semialdehyde Decarboxylase inhibited by 3-bromopropiolate==
-
<StructureSection load='4lho' size='340' side='right' caption='[[4lho]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
+
<StructureSection load='4lho' size='340' side='right'caption='[[4lho]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4lho]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LHO FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4lho]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LHO FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PR6:3-CHLORO-3-OXOPROPANOIC+ACID'>PR6</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.224&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lho OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lho RCSB], [http://www.ebi.ac.uk/pdbsum/4lho PDBsum]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PR6:3-CHLORO-3-OXOPROPANOIC+ACID'>PR6</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lho OCA], [https://pdbe.org/4lho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lho RCSB], [https://www.ebi.ac.uk/pdbsum/4lho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lho ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/F2Z288_9CORY F2Z288_9CORY]
-
Malonate semialdehyde decarboxylase from Pseudomonas pavonaceae 170 (designated Pp MSAD) is in a bacterial catabolic pathway for the nematicide 1,3-dichloropropene. MSAD has two known activities: it catalyzes the metal-ion independent decarboxylation of malonate semialdehyde to produce acetaldehyde and carbon dioxide, as well as a low-level hydration of 2-oxo-3-pentynoate to yield acetopyruvate. The latter activity is not known to be biologically relevant. Previous studies identified Pro-1, Asp-37, and a pair of arginines (Arg-73 and Arg-75) as critical residues in these activities. MSAD from Coryneform bacterium strain FG41 (designated FG41 MSAD) shares 38% pairwise sequence identity with the Pseudomonas enzyme including Pro-1 and Asp-37. However, Gln-73 replaces Arg-73, and the second arginine is shifted to Arg-76 by the insertion of a glycine. In order to determine how these changes relate to the activities of FG41 MSAD, the gene was cloned and the enzyme expressed and characterized. The enzyme has a comparable decarboxylase activity, but a significantly reduced hydratase activity. Mutagenesis along with crystal structures of the native enzyme (2.0 A resolution) and the enzyme modified by a 3-oxopropanoate moiety (resulting from the incubation of enzyme and 3-bromopropiolate) (2.2 A resolution) provided a structural basis. The roles of Pro-1 and Asp-37 are likely the same as those proposed for MSAD. However, the side chains of Thr-72, Gln-73, and Tyr-123 replace those of Arg-73 and Arg-75 in the mechanism and play a role in binding and catalysis. The structures also show that Arg-76 is likely too distant to play a direct role in the mechanism. FG41 MSAD is the second functionally annotated homologue in the MSAD family of the tautomerase superfamily and could represent a new subfamily.
+
-
 
+
-
Kinetic, Mutational, and Structural Analysis of Malonate Semialdehyde Decarboxylase from Coryneform bacterium strain FG41: Mechanistic Implications for the Decarboxylase and Hydratase Activities.,Guo Y, Serrano H, Poelarends GJ, Johnson WH, Hackert ML, Whitman CP Biochemistry. 2013 Jun 19. PMID:23781927<ref>PMID:23781927</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Coryneform bacterium]]
[[Category: Coryneform bacterium]]
-
[[Category: Guo, Y]]
+
[[Category: Large Structures]]
-
[[Category: Hackert, M L]]
+
[[Category: Guo Y]]
-
[[Category: Johnson, W H]]
+
[[Category: Hackert ML]]
-
[[Category: Poelarends, G J]]
+
[[Category: Johnson Jr WH]]
-
[[Category: Serrano, H]]
+
[[Category: Poelarends GJ]]
-
[[Category: Whitman, C P]]
+
[[Category: Serrano H]]
-
[[Category: Beta-alpha-beta-motif]]
+
[[Category: Whitman CP]]
-
[[Category: Isomerase-isomerase inhibitor complex]]
+
-
[[Category: Malonate semialdehyde decarboxylase]]
+
-
[[Category: The tautomerase superfamily]]
+

Current revision

Crystal Structure of FG41Malonate Semialdehyde Decarboxylase inhibited by 3-bromopropiolate

PDB ID 4lho

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4lho"
Personal tools