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5uja

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==Cryo-EM structure of bovine multidrug resistance protein 1 (MRP1) bound to leukotriene C4==
==Cryo-EM structure of bovine multidrug resistance protein 1 (MRP1) bound to leukotriene C4==
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<StructureSection load='5uja' size='340' side='right' caption='[[5uja]], [[Resolution|resolution]] 3.34&Aring;' scene=''>
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<SX load='5uja' size='340' side='right' viewer='molstar' caption='[[5uja]], [[Resolution|resolution]] 3.34&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5uja]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UJA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UJA FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5uja]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UJA FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LTX:(5~{S},6~{R},7~{E},9~{E},11~{Z},14~{Z})-6-[(2~{R})-2-[[(4~{S})-4-azanyl-5-oxidanyl-5-oxidanylidene-pentanoyl]amino]-3-(2-hydroxy-2-oxoethylamino)-3-oxidanylidene-propyl]sulfanyl-5-oxidanyl-icosa-7,9,11,14-tetraenoic+acid'>LTX</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.34&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LTX:(5~{S},6~{R},7~{E},9~{E},11~{Z},14~{Z})-6-[(2~{R})-2-[[(4~{S})-4-azanyl-5-oxidanyl-5-oxidanylidene-pentanoyl]amino]-3-(+2-hydroxy-2-oxoethylamino)-3-oxidanylidene-propyl]sulfanyl-5-oxidanyl-icosa-7,9,11,14-tetraenoic+acid'>LTX</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5uj9|5uj9]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uja OCA], [https://pdbe.org/5uja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uja RCSB], [https://www.ebi.ac.uk/pdbsum/5uja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uja ProSAT]</span></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ABCC1, MRP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uja OCA], [http://pdbe.org/5uja PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uja RCSB], [http://www.ebi.ac.uk/pdbsum/5uja PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uja ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/MRP1_BOVIN MRP1_BOVIN]
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The multidrug resistance protein MRP1 is an ATP-binding cassette (ABC) transporter that confers resistance to many anticancer drugs and plays a role in the disposition and efficacy of several opiates, antidepressants, statins, and antibiotics. In addition, MRP1 regulates redox homeostasis, inflammation, and hormone secretion. Using electron cryomicroscopy, we determined the molecular structures of bovine MRP1 in two conformations: an apo form at 3.5 A without any added substrate and a complex form at 3.3 A with one of its physiological substrates, leukotriene C4. These structures show that by forming a single bipartite binding site, MRP1 can recognize a spectrum of substrates with different chemical structures. We also observed large conformational changes induced by leukotriene C4, explaining how substrate binding primes the transporter for ATP hydrolysis. Structural comparison of MRP1 and P-glycoprotein advances our understanding of the common and unique properties of these two important molecules in multidrug resistance to chemotherapy.
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Structural Basis of Substrate Recognition by the Multidrug Resistance Protein MRP1.,Johnson ZL, Chen J Cell. 2017 Mar 9;168(6):1075-1085.e9. doi: 10.1016/j.cell.2017.01.041. Epub 2017 , Feb 23. PMID:28238471<ref>PMID:28238471</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5uja" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Bovin]]
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[[Category: Bos taurus]]
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[[Category: Chen, J]]
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[[Category: Large Structures]]
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[[Category: Johnson, Z E]]
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[[Category: Chen J]]
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[[Category: Abc transporter]]
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[[Category: Johnson ZL]]
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[[Category: Leukotriene c4]]
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[[Category: Ltc4]]
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[[Category: Multidrug resistance]]
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[[Category: Protein transport]]
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Current revision

Cryo-EM structure of bovine multidrug resistance protein 1 (MRP1) bound to leukotriene C4

5uja, resolution 3.34Å

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