This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5vjj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:10, 13 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='5vjj' size='340' side='right'caption='[[5vjj]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
<StructureSection load='5vjj' size='340' side='right'caption='[[5vjj]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5vjj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Melli Melli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VJJ FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5vjj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Melampsora_lini Melampsora lini]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VJJ FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vjj OCA], [http://pdbe.org/5vjj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vjj RCSB], [http://www.ebi.ac.uk/pdbsum/5vjj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vjj ProSAT]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vjj OCA], [https://pdbe.org/5vjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vjj RCSB], [https://www.ebi.ac.uk/pdbsum/5vjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vjj ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/B2ZCS6_MELLI B2ZCS6_MELLI]
-
The effector protein AvrP is secreted by the flax rust fungal pathogen (Melampsora lini) and recognized specifically by the flax (Linum usitatissimum) P disease resistance protein, leading to effector-triggered immunity. To investigate the biological function of this effector and mechanisms of specific recognition by the P resistance protein, we determined the crystal structure of AvrP. The structure reveals an elongated zinc-finger-like structure with a novel interleaved zinc-binding topology. The residues responsible for zinc binding are conserved in AvrP effector variants and mutations of these motifs result in loss of P-mediated recognition. The first zinc-coordinating region of the structure displays a positively-charged surface and has some limited similarities to nucleic acid-binding and chromatin-associated proteins. We show that the majority of the AvrP protein accumulates in the plant nucleus when transiently expressed in Nicotiana benthamiana cells, suggesting a nuclear pathogenic function. Polymorphic residues in AvrP and its allelic variants map to the protein surface and could be associated with differences in recognition specificity. Several point mutations of residues on the non-conserved surface patch result in a loss-of-recognition by P, suggesting that these residues are required for recognition. This article is protected by copyright. All rights reserved.
+
-
 
+
-
Crystal structure of the Melampsora lini effector AvrP reveals insights into a possible nuclear function and recognition by the flax disease resistance protein P.,Zhang X, Farah N, Rolston L, Ericsson DJ, Catanzariti AM, Bernoux M, Ve T, Bendak K, Chen C, Mackay JP, Lawrence GJ, Hardham A, Ellis JG, Williams SJ, Dodds PN, Jones DA, Kobe B Mol Plant Pathol. 2017 Aug 17. doi: 10.1111/mpp.12597. PMID:28817232<ref>PMID:28817232</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5vjj" style="background-color:#fffaf0;"></div>
+
==See Also==
==See Also==
*[[Avirulence protein 3D structures|Avirulence protein 3D structures]]
*[[Avirulence protein 3D structures|Avirulence protein 3D structures]]
-
== References ==
 
-
<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Melli]]
+
[[Category: Melampsora lini]]
-
[[Category: Ericsson, D J]]
+
[[Category: Ericsson DJ]]
-
[[Category: Kobe, B]]
+
[[Category: Kobe B]]
-
[[Category: Williams, S J]]
+
[[Category: Williams SJ]]
-
[[Category: Zhang, X]]
+
[[Category: Zhang X]]
-
[[Category: Metal binding protein]]
+
-
[[Category: Nuclear localisation]]
+
-
[[Category: Plant disease resistance]]
+
-
[[Category: Zinc finger]]
+

Current revision

Crystal structure of the flax-rust effector AvrP

PDB ID 5vjj

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5vjj"
Personal tools