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| | <StructureSection load='5vky' size='340' side='right'caption='[[5vky]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='5vky' size='340' side='right'caption='[[5vky]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5vky]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VKY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5vky]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_JAY291 Saccharomyces cerevisiae JAY291]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VKY FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TDA2, C1Q_03053 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=574961 Baker's yeast])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vky OCA], [http://pdbe.org/5vky PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vky RCSB], [http://www.ebi.ac.uk/pdbsum/5vky PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vky ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vky OCA], [https://pdbe.org/5vky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vky RCSB], [https://www.ebi.ac.uk/pdbsum/5vky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vky ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/TDA2_YEAS2 TDA2_YEAS2] |
| - | Clathrin- and actin-mediated endocytosis is essential in eukaryotic cells. In this study, we demonstrate that Tda2 is a novel protein of the endocytic machinery necessary for normal internalization of native cargo in yeast. Tda2 has not been classified in any protein family. Unexpectedly, solving the crystal structure of Tda2 revealed it belongs to the dynein light chain family. However, Tda2 works independently of the dynein motor complex and microtubules. Tda2 forms a tight complex with the polyproline motif-rich protein Aim21, which interacts physically with the SH3 domain of the Arp2/3 complex regulator Bbc1. The Tda2-Aim21 complex localizes to endocytic sites in a Bbc1- and filamentous actin-dependent manner. Importantly, the Tda2-Aim21 complex interacts directly with and facilitates the recruitment of actin-capping protein, revealing barbed-end filament capping at endocytic sites to be a regulated event. Thus, we have uncovered a new layer of regulation of the actin cytoskeleton by a member of a conserved protein family that has not been previously associated with a function in endocytosis.
| + | |
| - | | + | |
| - | Novel function of a dynein light chain in actin assembly during clathrin-mediated endocytosis.,Farrell KB, McDonald S, Lamb AK, Worcester C, Peersen OB, Di Pietro SM J Cell Biol. 2017 Aug 7;216(8):2565-2580. doi: 10.1083/jcb.201604123. Epub 2017, Jul 13. PMID:28706108<ref>PMID:28706108</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5vky" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: McDonald, S M]] | + | [[Category: Saccharomyces cerevisiae JAY291]] |
| - | [[Category: Peersen, O B]]
| + | [[Category: Di Pietro SM]] |
| - | [[Category: Pietro, S M.Di]] | + | [[Category: McDonald SM]] |
| - | [[Category: Dynein light chain clathrin mediated endocytosis]] | + | [[Category: Peersen OB]] |
| - | [[Category: Protein binding]] | + | |
