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5w7m

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==Crystal structure of RoqN==
==Crystal structure of RoqN==
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<StructureSection load='5w7m' size='340' side='right' caption='[[5w7m]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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<StructureSection load='5w7m' size='340' side='right'caption='[[5w7m]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5w7m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W7M FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5w7m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_rubens_Wisconsin_54-1255 Penicillium rubens Wisconsin 54-1255]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W7M FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w7m OCA], [http://pdbe.org/5w7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w7m RCSB], [http://www.ebi.ac.uk/pdbsum/5w7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w7m ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w7m OCA], [https://pdbe.org/5w7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w7m RCSB], [https://www.ebi.ac.uk/pdbsum/5w7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w7m ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ROQN_PENRW ROQN_PENRW]] Glandicoline B O-methyltransferase; part of the gene cluster that mediates the biosynthesis of the mycotoxin meleagrin (PubMed:22118684, PubMed:23776469). The first stage is catalyzed by the dipeptide synthase roqA which condenses histidine and tryptophan to produce histidyltryptophanyldiketopiperazine (HTD) (PubMed:22118684, PubMed:23776469). HTD is then converted to roquefortine C through two possible pathways (PubMed:23776469). In the first pathway, prenyltransferase roqD transforms HTD to the intermediate roquefortine D, which is in turn converted to roquefortine C by the cytochrome P450 monooxygenase roqR (PubMed:23776469). In the second pathway, HTD is first converted to the intermediate dehydrohistidyltryptophanyldi-ketopiperazine (DHTD) by roqR which is then prenylated by roqD to form roquefortine C (PubMed:23776469). Roquefortine C can be further transformed to meleagrin via three more reactions including oxydation to glandicolin A by roqM, which is further reduced to glandicoline B by roqO (PubMed:23776469). Finally, glandicoline B is converted to meleagrin by the glandicoline B O-methyltransferase roqN (PubMed:22118684, PubMed:23776469). More studies identified further branching and additional metabolites produced by the roquefortine/meleagrin cluster, including roquefortine F, roquefortine L, roquefortine M, roquefortine N and neoxaline (PubMed:24225953).<ref>PMID:22118684</ref> <ref>PMID:23776469</ref> <ref>PMID:24225953</ref>
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[https://www.uniprot.org/uniprot/ROQN_PENRW ROQN_PENRW] Glandicoline B O-methyltransferase; part of the gene cluster that mediates the biosynthesis of the mycotoxin meleagrin (PubMed:22118684, PubMed:23776469). The first stage is catalyzed by the dipeptide synthase roqA which condenses histidine and tryptophan to produce histidyltryptophanyldiketopiperazine (HTD) (PubMed:22118684, PubMed:23776469). HTD is then converted to roquefortine C through two possible pathways (PubMed:23776469). In the first pathway, prenyltransferase roqD transforms HTD to the intermediate roquefortine D, which is in turn converted to roquefortine C by the cytochrome P450 monooxygenase roqR (PubMed:23776469). In the second pathway, HTD is first converted to the intermediate dehydrohistidyltryptophanyldi-ketopiperazine (DHTD) by roqR which is then prenylated by roqD to form roquefortine C (PubMed:23776469). Roquefortine C can be further transformed to meleagrin via three more reactions including oxydation to glandicolin A by roqM, which is further reduced to glandicoline B by roqO (PubMed:23776469). Finally, glandicoline B is converted to meleagrin by the glandicoline B O-methyltransferase roqN (PubMed:22118684, PubMed:23776469). More studies identified further branching and additional metabolites produced by the roquefortine/meleagrin cluster, including roquefortine F, roquefortine L, roquefortine M, roquefortine N and neoxaline (PubMed:24225953).<ref>PMID:22118684</ref> <ref>PMID:23776469</ref> <ref>PMID:24225953</ref>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Berlinck, R G.S]]
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[[Category: Large Structures]]
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[[Category: Newmister, S A]]
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[[Category: Penicillium rubens Wisconsin 54-1255]]
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[[Category: Romminger, S]]
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[[Category: Berlinck RGS]]
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[[Category: Schmidt, J J]]
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[[Category: Newmister SA]]
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[[Category: Sherman, D H]]
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[[Category: Romminger S]]
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[[Category: Smith, J L]]
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[[Category: Schmidt JJ]]
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[[Category: Williams, R M]]
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[[Category: Sherman DH]]
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[[Category: Hydrolase]]
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[[Category: Smith JL]]
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[[Category: Indole alkaloid]]
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[[Category: Williams RM]]
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[[Category: Methyltransferase]]
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[[Category: Oxaline]]
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[[Category: Penicillium chrysogenum]]
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Current revision

Crystal structure of RoqN

PDB ID 5w7m

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