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5wc0

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katanin hexamer in spiral conformation

5wc0, resolution 4.40Å

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Retrieved from "http://52.214.119.220/wiki/index.php/5wc0"

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 <SX load='5wc0' size='340' side='right' viewer='molstar' caption='[[5wc0]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wc0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WC0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wc0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WC0 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.4&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wcb|5wcb]], [[5wc1|5wc1]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mei-1, T01G9.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wc0 OCA], [https://pdbe.org/5wc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wc0 RCSB], [https://www.ebi.ac.uk/pdbsum/5wc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wc0 ProSAT]</span></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microtubule-severing_ATPase Microtubule-severing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.3 3.6.4.3] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wc0 OCA], [http://pdbe.org/5wc0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wc0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wc0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wc0 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/KTNA1_CAEEL KTNA1_CAEEL]] Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays. Required specifically for meiotic spindle formation in the female germline; the presence of this protein is inimical to the formation of mitotic spindles (PubMed:8027178, PubMed:10809666, PubMed:12885567). In body wall muscles, regulates organization of myosin thick filaments (PubMed:22621901).[HAMAP-Rule:MF_03023]<ref>PMID:10809666</ref> <ref>PMID:12885567</ref> <ref>PMID:22621901</ref> <ref>PMID:8027178</ref>
+[https://www.uniprot.org/uniprot/KTNA1_CAEEL KTNA1_CAEEL] Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays. Required specifically for meiotic spindle formation in the female germline; the presence of this protein is inimical to the formation of mitotic spindles (PubMed:8027178, PubMed:10809666, PubMed:12885567). In body wall muscles, regulates organization of myosin thick filaments (PubMed:22621901).[HAMAP-Rule:MF_03023]<ref>PMID:10809666</ref> <ref>PMID:12885567</ref> <ref>PMID:22621901</ref> <ref>PMID:8027178</ref>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases important for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack of known 3D structures for these enzymes, their mechanism of action has remained poorly understood. Here we report the X-ray crystal structure of the monomeric AAA katanin module from Caenorhabditis elegans and cryo-EM reconstructions of the hexamer in two conformations. The structures reveal an unexpected asymmetric arrangement of the AAA domains mediated by structural elements unique to microtubule-severing enzymes and critical for their function. The reconstructions show that katanin cycles between open spiral and closed ring conformations, depending on the ATP occupancy of a gating protomer that tenses or relaxes interprotomer interfaces. Cycling of the hexamer between these conformations would provide the power stroke for microtubule severing.
-Katanin spiral and ring structures shed light on power stroke for microtubule severing.,Zehr E, Szyk A, Piszczek G, Szczesna E, Zuo X, Roll-Mecak A Nat Struct Mol Biol. 2017 Aug 7. doi: 10.1038/nsmb.3448. PMID:28783150<ref>PMID:28783150</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 5wc0" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </SX>
-[[Category: Caeel]]
+[[Category: Caenorhabditis elegans]]
 [[Category: Large Structures]]
-[[Category: Microtubule-severing ATPase]]
+[[Category: Piszczek G]]
-[[Category: Piszczek, G]]
+[[Category: Roll-Mecak A]]
-[[Category: Roll-Mecak, A]]
+[[Category: Szczesna E]]
-[[Category: Szczesna, E]]
+[[Category: Szyk A]]
-[[Category: Szyk, A]]
+[[Category: Zehr EA]]
-[[Category: Zehr, E A]]
+[[Category: Zuo X]]
-[[Category: Zuo, X]]
-[[Category: Aaa atpase]]
-[[Category: Microtubule cytoskeleton]]
-[[Category: Microtubule-severing enzyme]]
-[[Category: Motor protein]]
-[[Category: P60]]

5wc0

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katanin hexamer in spiral conformation

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