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6bxi

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==X-ray crystal structure of NDR1 kinase domain==
==X-ray crystal structure of NDR1 kinase domain==
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<StructureSection load='6bxi' size='340' side='right' caption='[[6bxi]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='6bxi' size='340' side='right'caption='[[6bxi]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6bxi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BXI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BXI FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6bxi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BXI FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bxi OCA], [http://pdbe.org/6bxi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bxi RCSB], [http://www.ebi.ac.uk/pdbsum/6bxi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bxi ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bxi OCA], [https://pdbe.org/6bxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bxi RCSB], [https://www.ebi.ac.uk/pdbsum/6bxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bxi ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/STK38_HUMAN STK38_HUMAN]] Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2.<ref>PMID:7761441</ref> <ref>PMID:12493777</ref> <ref>PMID:15197186</ref> <ref>PMID:17906693</ref>
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[https://www.uniprot.org/uniprot/STK38_HUMAN STK38_HUMAN] Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2.<ref>PMID:7761441</ref> <ref>PMID:12493777</ref> <ref>PMID:15197186</ref> <ref>PMID:17906693</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The human NDR family kinases control diverse aspects of cell growth, and are regulated through phosphorylation and association with scaffolds such as MOB1. Here, we report the crystal structure of the human NDR1 kinase domain in its non-phosphorylated state, revealing a fully resolved atypically long activation segment that blocks substrate binding and stabilizes a non-productive position of helix alphaC. Consistent with an auto-inhibitory function, mutations within the activation segment of NDR1 dramatically enhance in vitro kinase activity. Interestingly, NDR1 catalytic activity is further potentiated by MOB1 binding, suggesting that regulation through modulation of the activation segment and by MOB1 binding are mechanistically distinct. Lastly, deleting the auto-inhibitory activation segment of NDR1 causes a marked increase in the association with upstream Hippo pathway components and the Furry scaffold. These findings provide a point of departure for future efforts to explore the cellular functions and the mechanism of NDR1.
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Structural Basis for Auto-Inhibition of the NDR1 Kinase Domain by an Atypically Long Activation Segment.,Xiong S, Lorenzen K, Couzens AL, Templeton CM, Rajendran D, Mao DYL, Juang YC, Chiovitti D, Kurinov I, Guettler S, Gingras AC, Sicheri F Structure. 2018 Aug 7;26(8):1101-1115.e6. doi: 10.1016/j.str.2018.05.014. Epub, 2018 Jul 5. PMID:29983373<ref>PMID:29983373</ref>
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==See Also==
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*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6bxi" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Non-specific serine/threonine protein kinase]]
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[[Category: Homo sapiens]]
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[[Category: Sicheri, F]]
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[[Category: Large Structures]]
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[[Category: Xiong, S]]
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[[Category: Sicheri F]]
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[[Category: Kinase fold with atypically long activation segment]]
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[[Category: Xiong S]]
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[[Category: Transferase]]
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Current revision

X-ray crystal structure of NDR1 kinase domain

PDB ID 6bxi

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