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| | <StructureSection load='6cgo' size='340' side='right'caption='[[6cgo]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='6cgo' size='340' side='right'caption='[[6cgo]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cgo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burcm Burcm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CGO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cgo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_ambifaria_AMMD Burkholderia ambifaria AMMD]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CGO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Bamb_5915 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339670 BURCM])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cgo OCA], [http://pdbe.org/6cgo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cgo RCSB], [http://www.ebi.ac.uk/pdbsum/6cgo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cgo ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cgo OCA], [https://pdbe.org/6cgo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cgo RCSB], [https://www.ebi.ac.uk/pdbsum/6cgo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cgo ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q0B313_BURCM Q0B313_BURCM] |
| - | Modular polyketide synthases and non-ribosomal peptide synthetases are molecular assembly lines that consist of several multienzyme subunits that undergo dynamic self-assembly to form a functional megacomplex. N- and C-terminal docking domains are usually responsible for mediating the interactions between subunits. Here we show that communication between two non-ribosomal peptide synthetase subunits responsible for chain release from the enacyloxin polyketide synthase, which assembles an antibiotic with promising activity against Acinetobacter baumannii, is mediated by an intrinsically disordered short linear motif and a beta-hairpin docking domain. The structures, interactions and dynamics of these subunits were characterized using several complementary biophysical techniques to provide extensive insights into binding and catalysis. Bioinformatics analyses reveal that short linear motif/beta-hairpin docking domain pairs mediate subunit interactions in numerous non-ribosomal peptide and hybrid polyketide-non-ribosomal peptide synthetases, including those responsible for assembling several important drugs. Short linear motifs and beta-hairpin docking domains from heterologous systems are shown to interact productively, highlighting the potential of such interfaces as tools for biosynthetic engineering.
| + | |
| - | | + | |
| - | Structural basis for chain release from the enacyloxin polyketide synthase.,Kosol S, Gallo A, Griffiths D, Valentic TR, Masschelein J, Jenner M, de Los Santos ELC, Manzi L, Sydor PK, Rea D, Zhou S, Fulop V, Oldham NJ, Tsai SC, Challis GL, Lewandowski JR Nat Chem. 2019 Oct;11(10):913-923. doi: 10.1038/s41557-019-0335-5. Epub 2019 Sep , 23. PMID:31548674<ref>PMID:31548674</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6cgo" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Burcm]] | + | [[Category: Burkholderia ambifaria AMMD]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Challis, G L]] | + | [[Category: Challis GL]] |
| - | [[Category: Gallo, A]] | + | [[Category: De los Santos E]] |
| - | [[Category: Griffiths, D]] | + | [[Category: Gallo A]] |
| - | [[Category: Jenner, M]] | + | [[Category: Griffiths D]] |
| - | [[Category: Kosol, S]] | + | [[Category: Jenner M]] |
| - | [[Category: Lewandowski, J R]] | + | [[Category: Kosol S]] |
| - | [[Category: Masschelein, J L]] | + | [[Category: Lewandowski JR]] |
| - | [[Category: Santos, E De los]]
| + | [[Category: Masschelein JL]] |
| - | [[Category: Tsai, S C]] | + | [[Category: Tsai SC]] |
| - | [[Category: Valentic, T R]] | + | [[Category: Valentic TR]] |
| - | [[Category: Biosynthetic protein]]
| + | |
| - | [[Category: Nonribosomal peptide synthetase]]
| + | |
| - | [[Category: Polyketide]]
| + | |
