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6d6v

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==CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution==
==CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution==
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<StructureSection load='6d6v' size='340' side='right' caption='[[6d6v]], [[Resolution|resolution]] 4.80&Aring;' scene=''>
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<SX load='6d6v' size='340' side='right' viewer='molstar' caption='[[6d6v]], [[Resolution|resolution]] 4.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6d6v]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D6V FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6d6v]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D6V FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.8&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6v OCA], [https://pdbe.org/6d6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d6v RCSB], [https://www.ebi.ac.uk/pdbsum/6d6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6v ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6v OCA], [http://pdbe.org/6d6v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d6v RCSB], [http://www.ebi.ac.uk/pdbsum/6d6v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6v ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TERT_TETTH TERT_TETTH]] Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme (By similarity).
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[https://www.uniprot.org/uniprot/TEB1_TETTS TEB1_TETTS] Single-stranded DNA (ssDNA)-binding protein that mediates the recruitment of telomerase to telomeric DNA (PubMed:19941821, PubMed:20363756, PubMed:25225329, PubMed:22143754). Telomerase is an essential ribonucleoprotein (RNP) enzyme that copies new telomeric repeats onto chromosome ends by repetitively synthesizing the short telomere-repeat sequence 5'-TTGGGG-3' using an RNA template component TER (PubMed:19941821). Acts as part of a replication protein A (RPA)-related subcomplex of the holoenzyme telomerase ribonucleoprotein complex: TEB1 specifically recognizes and binds telomeric ssDNA, thereby mediating the recruitment of the holoenzyme telomerase RNP complex to telomeres (PubMed:19941821, PubMed:25225329, PubMed:27895115). TEB1 is related to RPA1 subunit of the RPA complex but is specific to telomeric DNA, which is not the case of RPA1 (PubMed:25225329).<ref>PMID:19941821</ref> <ref>PMID:20363756</ref> <ref>PMID:22143754</ref> <ref>PMID:25225329</ref> <ref>PMID:27895115</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Telomerase is an RNA-protein complex (RNP) that extends telomeric DNA at the 3' ends of chromosomes using its telomerase reverse transcriptase (TERT) and integral template-containing telomerase RNA (TER). Its activity is a critical determinant of human health, affecting aging, cancer, and stem cell renewal. Lack of atomic models of telomerase, particularly one with DNA bound, has limited our mechanistic understanding of telomeric DNA repeat synthesis. We report the 4.8 A resolution cryoelectron microscopy structure of active Tetrahymena telomerase bound to telomeric DNA. The catalytic core is an intricately interlocked structure of TERT and TER, including a previously structurally uncharacterized TERT domain that interacts with the TEN domain to physically enclose TER and regulate activity. This complete structure of a telomerase catalytic core and its interactions with telomeric DNA from the template to telomere-interacting p50-TEB complex provides unanticipated insights into telomerase assembly and catalytic cycle and a new paradigm for a reverse transcriptase RNP.
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Structure of Telomerase with Telomeric DNA.,Jiang J, Wang Y, Susac L, Chan H, Basu R, Zhou ZH, Feigon J Cell. 2018 May 17;173(5):1179-1190.e13. doi: 10.1016/j.cell.2018.04.038. PMID:29775593<ref>PMID:29775593</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6d6v" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Telomerase|Telomerase]]
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*[[Telomerase 3D structures|Telomerase 3D structures]]
*[[Telomerase-associated protein|Telomerase-associated protein]]
*[[Telomerase-associated protein|Telomerase-associated protein]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: RNA-directed DNA polymerase]]
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[[Category: Large Structures]]
[[Category: Tetrahymena thermophila]]
[[Category: Tetrahymena thermophila]]
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[[Category: Basu, R]]
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[[Category: Basu R]]
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[[Category: Chan, H]]
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[[Category: Chan H]]
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[[Category: Feigon, J]]
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[[Category: Feigon J]]
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[[Category: Jiang, J]]
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[[Category: Jiang J]]
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[[Category: Susac, L]]
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[[Category: Susac L]]
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[[Category: Wang, Y]]
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[[Category: Wang Y]]
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[[Category: Zhou, Z H]]
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[[Category: Zhou ZH]]
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[[Category: Replication]]
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[[Category: Telomerase]]
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[[Category: Telomere]]
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Current revision

CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution

6d6v, resolution 4.80Å

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