This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


6may

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:42, 13 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 3: Line 3:
<StructureSection load='6may' size='340' side='right'caption='[[6may]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
<StructureSection load='6may' size='340' side='right'caption='[[6may]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[6may]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MAY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MAY FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6may]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MAY FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=YNC:TETRADEC-13-YNOIC+ACID+-+COA+THIOESTER'>YNC</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
-
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycylpeptide_N-tetradecanoyltransferase Glycylpeptide N-tetradecanoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.97 2.3.1.97] </span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=YNC:TETRADEC-13-YNOIC+ACID+-+COA+THIOESTER'>YNC</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6may FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6may OCA], [http://pdbe.org/6may PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6may RCSB], [http://www.ebi.ac.uk/pdbsum/6may PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6may ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6may FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6may OCA], [https://pdbe.org/6may PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6may RCSB], [https://www.ebi.ac.uk/pdbsum/6may PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6may ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/A0A1G4HIY1_PLAVI A0A1G4HIY1_PLAVI]] Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.[RuleBase:RU000586]
+
[https://www.uniprot.org/uniprot/A0A1G4HIY1_PLAVI A0A1G4HIY1_PLAVI] Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.[RuleBase:RU000586]
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
The attachment of myristate to the N-terminal glycine of certain proteins is largely a co-translational modification catalyzed by N-myristoyltransferase (NMT), and involved in protein membrane-localization. Pathogen NMT is a validated therapeutic target in numerous infectious diseases including malaria. In Plasmodium falciparum, NMT substrates are important in essential processes including parasite gliding motility and host cell invasion. Here, we generated parasites resistant to a particular NMT inhibitor series and show that resistance in an in vitro parasite growth assay is mediated by a single amino acid substitution in the NMT substrate-binding pocket. The basis of resistance was validated and analyzed with a structure-guided approach using crystallography, in combination with enzyme activity, stability, and surface plasmon resonance assays, allowing identification of another inhibitor series unaffected by this substitution. We suggest that resistance studies incorporated early in the drug development process help selection of drug combinations to impede rapid evolution of parasite resistance.
+
-
 
+
-
Structure-Guided Identification of Resistance Breaking Antimalarial NMyristoyltransferase Inhibitors.,Schlott AC, Mayclin S, Reers AR, Coburn-Flynn O, Bell AS, Green J, Knuepfer E, Charter D, Bonnert R, Campo B, Burrows J, Lyons-Abbott S, Staker BL, Chung CW, Myler PJ, Fidock DA, Tate EW, Holder AA Cell Chem Biol. 2019 Apr 9. pii: S2451-9456(19)30111-4. doi:, 10.1016/j.chembiol.2019.03.015. PMID:31080074<ref>PMID:31080074</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 6may" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Glycylpeptide N-tetradecanoyltransferase]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Structural genomic]]
 
-
[[Category: Glycylpeptide n-tetradecanoyltransferase]]
 
-
[[Category: N-myristoyltransferase]]
 
-
[[Category: Nmt]]
 
[[Category: Plasmodium vivax]]
[[Category: Plasmodium vivax]]
-
[[Category: Salvador i]]
 
-
[[Category: Ssgcid]]
 
-
[[Category: Transferase]]
 

Current revision

Crystal structure of N-myristoyl transferase (NMT) G386E mutant from Plasmodium vivax

PDB ID 6may

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6may"
Personal tools