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6mec

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==Structure of a group II intron retroelement after DNA integration==
==Structure of a group II intron retroelement after DNA integration==
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<StructureSection load='6mec' size='340' side='right'caption='[[6mec]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
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<SX load='6mec' size='340' side='right' viewer='molstar' caption='[[6mec]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6mec]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Theeb Theeb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MEC FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6mec]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus Thermosynechococcus vestitus] and [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MEC FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tll0114 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mec OCA], [http://pdbe.org/6mec PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mec RCSB], [http://www.ebi.ac.uk/pdbsum/6mec PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mec ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mec OCA], [https://pdbe.org/6mec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mec RCSB], [https://www.ebi.ac.uk/pdbsum/6mec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mec ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8DMK2_THEVB Q8DMK2_THEVB]
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Group II introns are a class of retroelements that invade DNA through a copy-and-paste mechanism known as retrotransposition. Their coordinated activities occur within a complex that includes a maturase protein, which promotes splicing through an unknown mechanism. The mechanism of splice site exchange within the RNA active site during catalysis also remains unclear. We determined two cryo-EM structures at 3.6-A resolution of a group II intron reverse splicing into DNA. These structures reveal that the branch-site domain VI helix swings 90 degrees , enabling substrate exchange during DNA integration. The maturase assists catalysis through a transient RNA-protein contact with domain VI that positions the branch-site adenosine for lariat formation during forward splicing. These findings provide the first direct evidence of the role the maturase plays during group II intron catalysis. The domain VI dynamics closely parallel spliceosomal branch-site helix movement and provide strong evidence for a retroelement origin of the spliceosome.
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Cryo-EM Structures of a Group II Intron Reverse Splicing into DNA.,Haack DB, Yan X, Zhang C, Hingey J, Lyumkis D, Baker TS, Toor N Cell. 2019 Jul 25;178(3):612-623.e12. doi: 10.1016/j.cell.2019.06.035. PMID:31348888<ref>PMID:31348888</ref>
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==See Also==
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*[[Reverse transcriptase 3D structures|Reverse transcriptase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6mec" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Theeb]]
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[[Category: Thermosynechococcus vestitus]]
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[[Category: Baker, T S]]
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[[Category: Thermosynechococcus vestitus BP-1]]
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[[Category: Haack, D]]
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[[Category: Baker TS]]
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[[Category: Hingey, J]]
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[[Category: Haack D]]
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[[Category: Lyumkis, D]]
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[[Category: Hingey J]]
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[[Category: Toor, N]]
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[[Category: Lyumkis D]]
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[[Category: Yan, X]]
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[[Category: Toor N]]
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[[Category: Zhang, C]]
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[[Category: Yan X]]
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[[Category: Group ii intron]]
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[[Category: Zhang C]]
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[[Category: Retroelement]]
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[[Category: Retrotransposition]]
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[[Category: Rna-dna-rna binding protein complex]]
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Current revision

Structure of a group II intron retroelement after DNA integration

6mec, resolution 3.60Å

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