6nmg

<table><tr><td colspan='2'>[[6nmg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NMG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NMG FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ric8a, rCG_48458 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nmg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nmg OCA], [http://pdbe.org/6nmg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nmg RCSB], [http://www.ebi.ac.uk/pdbsum/6nmg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nmg ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Ric-8A is a 530-amino acid cytoplasmic molecular chaperone and guanine nucleotide exchange factor (GEF) for i, q, and 12/13 classes of heterortrimeric G protein alpha subunits (Galpha). We report the 2.2-A crystal structure of the Ric-8A Galpha-binding domain with GEF activity, residues 1-452, and is phosphorylated at Ser435 and Thr440. Residues 1-429 adopt a superhelical fold comprised of Armadillo (ARM) and HEAT repeats, and the C terminus is disordered. One of the phosphorylated residues potentially binds to a basic cluster in an ARM motif. Amino acid sequence conservation and published hydrogen-deuterium exchange data indicate repeats 3 through 6 to be a putative Galpha-binding surface. Normal mode modeling of small-angle X-ray scattering data indicates that phosphorylation induces relative rotation between repeats 1-4, 5-6, and 7-9. 2D (1)H-(15)N-TROSY spectra of [(2)H,(15)N]-labeled Galphai1 in the presence of R452 reveals chemical shift perturbations of the C terminus and Galphai1 residues involved in nucleotide binding.

 

Structure, Function, and Dynamics of the Galpha Binding Domain of Ric-8A.,Zeng B, Mou TC, Doukov TI, Steiner A, Yu W, Papasergi-Scott M, Tall GG, Hagn F, Sprang SR Structure. 2019 Jul 2;27(7):1137-1147.e5. doi: 10.1016/j.str.2019.04.013. Epub, 2019 May 30. PMID:31155309<ref>PMID:31155309</ref>

 

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 6nmg" style="background-color:#fffaf0;"></div>

[[Category: Buffalo rat]]

[[Category: Mou, T C]]

[[Category: Sprang, S R]]

[[Category: Zeng, B]]

[[Category: Signaling protein]]