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| | <SX load='6od7' size='340' side='right' viewer='molstar' caption='[[6od7]], [[Resolution|resolution]] 5.60Å' scene=''> | | <SX load='6od7' size='340' side='right' viewer='molstar' caption='[[6od7]], [[Resolution|resolution]] 5.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6od7]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_1_strain_kos Human herpesvirus 1 strain kos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OD7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OD7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6od7]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1_strain_KOS Human alphaherpesvirus 1 strain KOS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OD7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OD7 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6od7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6od7 OCA], [http://pdbe.org/6od7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6od7 RCSB], [http://www.ebi.ac.uk/pdbsum/6od7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6od7 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.6Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6od7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6od7 OCA], [https://pdbe.org/6od7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6od7 RCSB], [https://www.ebi.ac.uk/pdbsum/6od7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6od7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/H9E912_HHV1 H9E912_HHV1]] Forms a portal in the viral capsid through which viral DNA is translocated during DNA packaging. Assembles as a dodecamer at a single fivefold axe of the T=16 icosahedric capsid. Binds to the molecular motor that translocates the viral DNA, termed terminase.[HAMAP-Rule:MF_04012] | + | [https://www.uniprot.org/uniprot/H9E912_HHV1 H9E912_HHV1] Forms a portal in the viral capsid through which viral DNA is translocated during DNA packaging. Assembles as a dodecamer at a single fivefold axe of the T=16 icosahedric capsid. Binds to the molecular motor that translocates the viral DNA, termed terminase.[HAMAP-Rule:MF_04012] |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Herpesviruses are enveloped viruses that are prevalent in the human population and are responsible for diverse pathologies, including cold sores, birth defects and cancers. They are characterized by a highly pressurized pseudo-icosahedral capsid-with triangulation number (T) equal to 16-encapsidating a tightly packed double-stranded DNA (dsDNA) genome(1-3). A key process in the herpesvirus life cycle involves the recruitment of an ATP-driven terminase to a unique portal vertex to recognize, package and cleave concatemeric dsDNA, ultimately giving rise to a pressurized, genome-containing virion(4,5). Although this process has been studied in dsDNA phages(6-9)-with which herpesviruses bear some similarities-a lack of high-resolution in situ structures of genome-packaging machinery has prevented the elucidation of how these multi-step reactions, which require close coordination among multiple actors, occur in an integrated environment. To better define the structural basis of genome packaging and organization in herpes simplex virus type 1 (HSV-1), we developed sequential localized classification and symmetry relaxation methods to process cryo-electron microscopy (cryo-EM) images of HSV-1 virions, which enabled us to decouple and reconstruct hetero-symmetric and asymmetric elements within the pseudo-icosahedral capsid. Here we present in situ structures of the unique portal vertex, genomic termini and ordered dsDNA coils in the capsid spooled around a disordered dsDNA core. We identify tentacle-like helices and a globular complex capping the portal vertex that is not observed in phages, indicative of herpesvirus-specific adaptations in the DNA-packaging process. Finally, our atomic models of portal vertex elements reveal how the fivefold-related capsid accommodates symmetry mismatch imparted by the dodecameric portal-a longstanding mystery in icosahedral viruses-and inform possible DNA-sequence recognition and headful-sensing pathways involved in genome packaging. This work showcases how to resolve symmetry-mismatched elements in a large eukaryotic virus and provides insights into the mechanisms of herpesvirus genome packaging.
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| - | | + | |
| - | Cryo-EM structures of herpes simplex virus type 1 portal vertex and packaged genome.,Liu YT, Jih J, Dai X, Bi GQ, Zhou ZH Nature. 2019 Jun;570(7760):257-261. doi: 10.1038/s41586-019-1248-6. Epub 2019 May, 29. PMID:31142842<ref>PMID:31142842</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 6od7" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Portal protein|Portal protein]] | + | *[[Portal protein 3D structures|Portal protein 3D structures]] |
| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Human herpesvirus 1 strain kos]] | + | [[Category: Human alphaherpesvirus 1 strain KOS]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bi, G Q]] | + | [[Category: Bi GQ]] |
| - | [[Category: Dai, X H]] | + | [[Category: Dai XH]] |
| - | [[Category: Jih, J]] | + | [[Category: Jih J]] |
| - | [[Category: Liu, Y T]] | + | [[Category: Liu YT]] |
| - | [[Category: Zhou, Z H]] | + | [[Category: Zhou ZH]] |
| - | [[Category: Dna-packaging]]
| + | |
| - | [[Category: Dna-translocation]]
| + | |
| - | [[Category: Dodecamer]]
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| - | [[Category: Portal]]
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| - | [[Category: Viral protein]]
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