1a5b

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(New page: 200px<br /> <applet load="1a5b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a5b, resolution 2.0&Aring;" /> '''CRYO-CRYSTALLOGRAPHY...)
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[[Image:1a5b.gif|left|200px]]<br />
 
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<applet load="1a5b" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a5b, resolution 2.0&Aring;" />
 
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'''CRYO-CRYSTALLOGRAPHY OF A TRUE SUBSTRATE, INDOLE-3-GLYCEROL PHOSPHATE, BOUND TO A MUTANT (ALPHA D60N) TRYPTOPHAN SYNTHASE ALPHA2BETA2 COMPLEX REVEALS THE CORRECT ORIENTATION OF ACTIVE SITE ALPHA GLU 49'''<br />
 
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==Overview==
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==CRYO-CRYSTALLOGRAPHY OF A TRUE SUBSTRATE, INDOLE-3-GLYCEROL PHOSPHATE, BOUND TO A MUTANT (ALPHA D60N) TRYPTOPHAN SYNTHASE ALPHA2BETA2 COMPLEX REVEALS THE CORRECT ORIENTATION OF ACTIVE SITE ALPHA GLU 49==
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The reversible cleavage of indole-3-glycerol by the alpha-subunit of, tryptophan synthase has been proposed to be catalyzed by alphaGlu49 and, alphaAsp60. Although previous x-ray crystallographic structures of the, tryptophan synthase alpha2beta2 complex showed an interaction between the, carboxylate of alphaAsp60 and the bound inhibitor indole-3-propanol, phosphate, the carboxylate of alphaGlu49 was too distant to play its, proposed role. To clarify the structural and functional roles of, alphaGlu49, we have determined crystal structures of a mutant (alphaD60N), alpha2beta2 complex in the presence and absence of the true substrate, indole-3-glycerol phosphate. The enzyme in the crystal cleaves, indole-3-glycerol phosphate very slowly at room temperature but not under, cryo-conditions of ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9535826 (full description)]]
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<StructureSection load='1a5b' size='340' side='right'caption='[[1a5b]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1a5b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A5B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IGP:INDOLE-3-GLYCEROL+PHOSPHATE'>IGP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a5b OCA], [https://pdbe.org/1a5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a5b RCSB], [https://www.ebi.ac.uk/pdbsum/1a5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a5b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRPA_SALTY TRPA_SALTY] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/1a5b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a5b ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1A5B is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with K, IGP and PLP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A5B OCA]].
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*[[Tryptophan synthase 3D structures|Tryptophan synthase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Cryo-crystallography of a true substrate, indole-3-glycerol phosphate, bound to a mutant (alphaD60N) tryptophan synthase alpha2beta2 complex reveals the correct orientation of active site alphaGlu49., Rhee S, Miles EW, Davies DR, J Biol Chem. 1998 Apr 10;273(15):8553-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9535826 9535826]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Salmonella typhimurium]]
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[[Category: Davies DR]]
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[[Category: Davies, D.R.]]
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[[Category: Miles EW]]
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[[Category: Miles, E.W.]]
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[[Category: Rhee S]]
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[[Category: Rhee, S.]]
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[[Category: IGP]]
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[[Category: K]]
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[[Category: PLP]]
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[[Category: carbon-oxygen lyase]]
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[[Category: mutation at position 60 (asp --> asn) in the a-subunit]]
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[[Category: true substrate indole-3-glycerol phosphate in the a-subunit]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:27:08 2007''
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Current revision

CRYO-CRYSTALLOGRAPHY OF A TRUE SUBSTRATE, INDOLE-3-GLYCEROL PHOSPHATE, BOUND TO A MUTANT (ALPHA D60N) TRYPTOPHAN SYNTHASE ALPHA2BETA2 COMPLEX REVEALS THE CORRECT ORIENTATION OF ACTIVE SITE ALPHA GLU 49

PDB ID 1a5b

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