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1ahl

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(New page: 200px<br /><applet load="1ahl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ahl" /> '''ANTHOPLEURIN-A,NMR, 20 STRUCTURES'''<br /> ...)
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[[Image:1ahl.gif|left|200px]]<br /><applet load="1ahl" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ahl" />
 
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'''ANTHOPLEURIN-A,NMR, 20 STRUCTURES'''<br />
 
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==Overview==
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==ANTHOPLEURIN-A,NMR, 20 STRUCTURES==
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The three-dimensional structure in aqueous solution of the 49-residue, polypeptide anthopleurin-A (AP-A), from the sea anemone Anthopleura, xanthogrammica, has been determined from 1H NMR data. A restraint set, consisting of 411 interproton distance restraints inferred from NOEs and, 19 backbone and 13 side chain dihedral angle restraints from spin-spin, coupling constants, as well as 15 lower bound restraints based on the, absence of NOEs in the spectra, was used as input for distance geometry, calculations in DIANA and simulated annealing and restrained energy, minimization in X-PLOR. Stereospecific assignments for 12 beta-methylene, pairs were also included. The final set of 20 structures had mean pairwise, rms differences over the whole molecule of 2.04 A for the backbone heavy, atoms (N, C alpha, and C) and 2.59 A for all heavy atoms. For the, well-defined region encompassing residues 2-7 and 17-49, the corresponding, values were 0.82 and 1.27 A, respectively. AP-A adopts a compact structure, consisting of four short strands of antiparallel beta-sheet (residues 2-4, 20-23, 34-37, and 45-48) connected by three loops. The first loop, commences with a type I beta-turn which includes two important Asp, residues; this loop is the least well-defined region of the protein, although a beta-turn involving residues 13-16 is observed in nearly half, the structures. The loop linking the second and third strands is, constrained by the 29-47 disulfide bond and contains two well-defined, beta-turns, while the third loop contains the Gly40-Pro41 sequence, which, has been identified previously as the site of cis-trans isomerism. The, carboxylate group of Asp7 is close to the epsilon-ammonium group of Lys37, suggesting that they may form a salt bridge. A pH titration monitored by, 2D NMR supports this by showing that Asp7 has a low pKa. It is proposed, that this region of the molecule and the nearby residues Asp9 and His39, form part of the molecular surface which interacts with the mammalian, cardiac sodium channel.
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<StructureSection load='1ahl' size='340' side='right'caption='[[1ahl]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ahl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anthopleura_xanthogrammica Anthopleura xanthogrammica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AHL FirstGlance]. <br>
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1AHL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anthopleura_xanthogrammica Anthopleura xanthogrammica]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AHL OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ahl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahl OCA], [https://pdbe.org/1ahl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ahl RCSB], [https://www.ebi.ac.uk/pdbsum/1ahl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ahl ProSAT]</span></td></tr>
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==Reference==
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</table>
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Three-dimensional structure in solution of the polypeptide cardiac stimulant anthopleurin-A., Pallaghy PK, Scanlon MJ, Monks SA, Norton RS, Biochemistry. 1995 Mar 21;34(11):3782-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7893675 7893675]
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== Function ==
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[https://www.uniprot.org/uniprot/NA1A_ANTXA NA1A_ANTXA] Binds specifically to voltage-gated sodium channels (Nav) (site 3), thereby delaying their inactivation. This toxin retains the greatest capacity to discriminate between the cardiac (Nav1.5/SCN5A) and neuronal sodium channels (2.5 nM versus 120 nM, when electrophysiologically tested and 14 nM versus 400 nM, when tested by ion flux), whereas its paralog Anthopleurin-B has the highest affinity of all anemone toxins for the mammalian sodium channel (PubMed:13806, PubMed:7612595, PubMed:17092528). Its ability to differentiate between cardiac and skeletal channels appears to be associated with domain 4 of the channel (PubMed:9306007). This toxin does not slow or inhibit closed-state inactivation of cardiac sodium channels, but selectively modifies inactivation from the open-state (PubMed:8576699). It does not display phospholipid-binding activities, suggesting that the domain IV S3-S4 linker is located at the extracellular surface and not buried in the phospholipid bilayer (By similarity).[UniProtKB:P01531]<ref>PMID:13806</ref> <ref>PMID:17092528</ref> <ref>PMID:21099342</ref> <ref>PMID:8576699</ref> <ref>PMID:9306007</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/1ahl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ahl ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Anthopleura xanthogrammica]]
[[Category: Anthopleura xanthogrammica]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Monks, S.A.]]
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[[Category: Monks SA]]
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[[Category: Norton, R.S.]]
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[[Category: Norton RS]]
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[[Category: Pallaghy, P.K.]]
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[[Category: Pallaghy PK]]
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[[Category: Scanlon, M.J.]]
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[[Category: Scanlon MJ]]
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[[Category: cardiac stimulant]]
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[[Category: neurotoxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:51:46 2007''
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ANTHOPLEURIN-A,NMR, 20 STRUCTURES

PDB ID 1ahl

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