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1ais

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(New page: 200px<br /><applet load="1ais" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ais, resolution 2.100&Aring;" /> '''TATA-BINDING PROTEI...)
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[[Image:1ais.jpg|left|200px]]<br /><applet load="1ais" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ais, resolution 2.100&Aring;" />
 
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'''TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI'''<br />
 
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==Overview==
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==TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI==
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Archaea possess a basal transcriptional apparatus that resembles that of, eukaryotes. Here we report the 2.1-A crystal structure of the archaeal, transcription factor complex formed by the TATA-box-binding protein (TBP), the transcription factor IIB homolog, and a DNA target, all from the, hyperthermophile Pyrococcus woesei. The overall fold of these two basal, transcription factors is essentially the same as that of their eukaryotic, counterparts. However, in comparison with the eukaryotic complexes, the, archaeal TBP-DNA interface is more symmetrical, and in this structure the, orientation of the preinitiation complex assembly on the promoter is, inverted with respect to that seen in all crystal structures of comparable, eukaryotic systems. This study of the structural details of an archaeal, transcription factor complex presents the opportunity to examine the, evolution of the basal eukaryotic transcriptional apparatus from a, stereochemical viewpoint and to extend our understanding of the physical, biochemistry of transcriptional initiation.
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<StructureSection load='1ais' size='340' side='right'caption='[[1ais]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ais]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AIS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ais FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ais OCA], [https://pdbe.org/1ais PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ais RCSB], [https://www.ebi.ac.uk/pdbsum/1ais PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ais ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TBP_PYRWO TBP_PYRWO] General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/1ais_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ais ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AIS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AIS OCA].
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*[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]]
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*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
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==Reference==
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__TOC__
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The 2.1-A crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box., Kosa PF, Ghosh G, DeDecker BS, Sigler PB, Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6042-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9177165 9177165]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Pyrococcus woesei]]
[[Category: Pyrococcus woesei]]
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[[Category: Dedecker, B.S.]]
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[[Category: Dedecker BS]]
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[[Category: Ghosh, G.]]
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[[Category: Ghosh G]]
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[[Category: Kosa, P.F.]]
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[[Category: Kosa PF]]
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[[Category: Sigler, P.B.]]
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[[Category: Sigler PB]]
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[[Category: complex (ribosome binding/ dna)]]
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[[Category: hyperthermophile]]
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[[Category: ribosome binding]]
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[[Category: transcription]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:53:22 2007''
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Current revision

TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI

PDB ID 1ais

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