This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1aur

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:28, 13 March 2024) (edit) (undo)
 
(22 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1aur.gif|left|200px]]<br />
 
-
<applet load="1aur" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1aur, resolution 2.5&Aring;" />
 
-
'''PMSF-INHIBITED CARBOXYLESTERASE FROM PSEUDOMONAS FLUORESCENS'''<br />
 
-
==Overview==
+
==PMSF-INHIBITED CARBOXYLESTERASE FROM PSEUDOMONAS FLUORESCENS==
-
BACKGROUND: A group of esterases, classified as carboxylesterases, hydrolyze carboxylic ester bonds with relatively broad substrate, specificity and are useful for stereospecific synthesis and hydrolysis of, esters. One such carboxylesterase from Pseudomonas fluorescens is a, homodimeric enzyme, consisting of 218-residue subunits. It shows a limited, sequence similarity to some members of the alpha/beta hydrolase, superfamily. Although crystal structures of a number of serine esterases, and lipases have been reported, structural information on, carboxylesterases is very limited. This study was undertaken in order to, provide such information and to understand a structural basis for the, substrate specificity of this carboxylesterase. RESULTS: In this study, the crystal structure of ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9438866 (full description)]]
+
<StructureSection load='1aur' size='340' side='right'caption='[[1aur]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1aur]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AUR FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aur OCA], [https://pdbe.org/1aur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aur RCSB], [https://www.ebi.ac.uk/pdbsum/1aur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aur ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/EST2_PSEFL EST2_PSEFL] Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/1aur_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aur ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1AUR is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]] with PMS as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1]]. Structure known Active Sites: ACA and ACB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AUR OCA]].
+
*[[Carboxylesterase 3D structures|Carboxylesterase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structure of carboxylesterase from Pseudomonas fluorescens, an alpha/beta hydrolase with broad substrate specificity., Kim KK, Song HK, Shin DH, Hwang KY, Choe S, Yoo OJ, Suh SW, Structure. 1997 Dec 15;5(12):1571-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9438866 9438866]
+
[[Category: Large Structures]]
-
[[Category: Carboxylesterase]]
+
[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
-
[[Category: Single protein]]
+
[[Category: Kim KK]]
-
[[Category: Kim, K.K.]]
+
[[Category: Song HK]]
-
[[Category: Song, H.K.]]
+
[[Category: Suh SW]]
-
[[Category: Suh, S.W.]]
+
-
[[Category: PMS]]
+
-
[[Category: hydrolase]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:51:35 2007''
+

Current revision

PMSF-INHIBITED CARBOXYLESTERASE FROM PSEUDOMONAS FLUORESCENS

PDB ID 1aur

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1aur"
Personal tools