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1awt

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[[Image:1awt.gif|left|200px]]<br />
 
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<applet load="1awt" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1awt, resolution 2.55&Aring;" />
 
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'''SECYPA COMPLEXED WITH HAGPIA'''<br />
 
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==Overview==
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==SECYPA COMPLEXED WITH HAGPIA==
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The cellular protein, cyclophilin A (CypA), is incorporated into the, virion of the type 1 human immunodeficiency virus (HIV-1) via a direct, interaction with the capsid domain of the viral Gag polyprotein. We, demonstrate that the capsid sequence 87His-Ala-Gly-Pro-Ile-Ala92, (87HAGPIA92) encompasses the primary cyclophilin A binding site and, present an X-ray crystal structure of the CypA/HAGPIA complex. In contrast, to the cis prolines observed in all previously reported structures of CypA, complexed with model peptides, the proline in this peptide, Pro 90, binds, the cyclophilin A active site in a trans conformation. We also report the, crystal structure of a complex between CypA and the hexapeptide HVGPIA, which also maintains the trans conformation. Comparison with the recently, determined structures of CypA in complexes with larger fragments of the, HIV-1 capsid protein demonstrates that CypA recognition of these, hexapeptides involves contacts with peptide residues Ala(Val) 88, Gly 89, and Pro 90, and is independent of the context of longer sequences.
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<StructureSection load='1awt' size='340' side='right'caption='[[1awt]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1awt]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AWT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1awt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1awt OCA], [https://pdbe.org/1awt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1awt RCSB], [https://www.ebi.ac.uk/pdbsum/1awt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1awt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PPIA_HUMAN PPIA_HUMAN] PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aw/1awt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1awt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AWT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AWT OCA].
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*[[Cyclophilin 3D structures|Cyclophilin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of cyclophilin A complexed with a binding site peptide from the HIV-1 capsid protein., Vajdos FF, Yoo S, Houseweart M, Sundquist WI, Hill CP, Protein Sci. 1997 Nov;6(11):2297-307. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9385632 9385632]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Peptidylprolyl isomerase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Vajdos FF]]
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[[Category: Vajdos, F.F.]]
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[[Category: complex (isomerase/peptide)]]
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[[Category: cyclophilin a]]
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[[Category: hiv-1 capsid]]
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[[Category: pseudo-symmetry]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:03:11 2007''
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Current revision

SECYPA COMPLEXED WITH HAGPIA

PDB ID 1awt

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