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1axh

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(New page: 200px<br /><applet load="1axh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1axh" /> '''ATRACOTOXIN-HVI FROM HADRONYCHE VERSUTA (AUS...)
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[[Image:1axh.gif|left|200px]]<br /><applet load="1axh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1axh" />
 
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'''ATRACOTOXIN-HVI FROM HADRONYCHE VERSUTA (AUSTRALIAN FUNNEL-WEB SPIDER, NMR, 20 STRUCTURES'''<br />
 
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==Overview==
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==ATRACOTOXIN-HVI FROM HADRONYCHE VERSUTA (AUSTRALIAN FUNNEL-WEB SPIDER, NMR, 20 STRUCTURES==
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A family of potent insecticidal toxins has recently been isolated from the, venom of Australian funnel web spiders. Among these is the 37-residue, peptide omega-atracotoxin-HV1 (omega-ACTX-HV1) from Hadronyche versuta. We, have chemically synthesized and folded omega-ACTX-HV1, shown that it is, neurotoxic, ascertained its disulphide bonding pattern, and determined its, three-dimensional solution structure using NMR spectroscopy. The structure, consists of a solvent-accessible beta-hairpin protruding from a, disulphide-bonded globular core comprising four beta-turns. The three, intramolecular disulphide bonds from a cystine knot motif similar to that, seen in several other neurotoxic peptides. Despite limited sequence, identity, omega-ACTX-HV1 displays significant structural homology with the, omega-agatoxins and omega-conotoxins, both of which are vertebrate calcium, channel antagonists; however, in contrast with these toxins, we show that, omega-ACTX-HV1 inhibits insect, but not mammalian, voltage-gated calcium, channel currents.
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<StructureSection load='1axh' size='340' side='right'caption='[[1axh]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1axh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hadronyche_versuta Hadronyche versuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AXH FirstGlance]. <br>
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1AXH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hadronyche_versuta Hadronyche versuta]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AXH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1axh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1axh OCA], [https://pdbe.org/1axh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1axh RCSB], [https://www.ebi.ac.uk/pdbsum/1axh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1axh ProSAT]</span></td></tr>
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==Reference==
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</table>
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The structure of a novel insecticidal neurotoxin, omega-atracotoxin-HV1, from the venom of an Australian funnel web spider., Fletcher JI, Smith R, O'Donoghue SI, Nilges M, Connor M, Howden ME, Christie MJ, King GF, Nat Struct Biol. 1997 Jul;4(7):559-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9228949 9228949]
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== Function ==
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[https://www.uniprot.org/uniprot/TO1A_HADVE TO1A_HADVE] Reversibly and voltage-independently blocks both mid-low- (M-LVA) and high-voltage-activated (HVA) calcium channels in cockroach DUM neurons (PubMed:17610847). Is lethal to many insect species but not toxic to mammals (PubMed:9228949, PubMed:16779650). May target the insect high-voltage-activated calcium channel Dmca1D. Also inhibits acarines calcium channels. An extremely high toxin concentration partially inhibits Cav1.2/CACNA1C, Cav2.1/CACNA1A and Cav2.2/CACNA1B calcium channel of rats. As for omega-AcTx-Hv2a, the phenotypic effect of injection of this toxin into lone star ticks (Amblyomma americanum) is curling of all eight legs into closed loops.<ref>PMID:14608494</ref> <ref>PMID:15308644</ref> <ref>PMID:16330063</ref> <ref>PMID:17141372</ref> <ref>PMID:17610847</ref> <ref>PMID:9228949</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ax/1axh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1axh ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Hadronyche versuta]]
[[Category: Hadronyche versuta]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Donoghue, S.I.O.]]
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[[Category: Fletcher JI]]
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[[Category: Fletcher, J.I.]]
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[[Category: King GF]]
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[[Category: King, G.F.]]
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[[Category: Nilges M]]
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[[Category: Nilges, M.]]
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[[Category: O'Donoghue SI]]
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[[Category: cystine knot]]
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[[Category: funnel-web]]
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[[Category: insecticidal toxin]]
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[[Category: neurotoxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:11:13 2007''
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Current revision

ATRACOTOXIN-HVI FROM HADRONYCHE VERSUTA (AUSTRALIAN FUNNEL-WEB SPIDER, NMR, 20 STRUCTURES

PDB ID 1axh

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