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1bfa

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[[Image:1bfa.gif|left|200px]]<br />
 
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<applet load="1bfa" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bfa, resolution 2.20&Aring;" />
 
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'''RECOMBINANT BIFUNCTIONAL HAGEMAN FACTOR/AMYLASE INHIBITOR FROM MAIZE'''<br />
 
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==Overview==
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==RECOMBINANT BIFUNCTIONAL HAGEMAN FACTOR/AMYLASE INHIBITOR FROM MAIZE==
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Corn Hageman factor inhibitor (CHFI) is a bifunctional 127 residue, 13.6, kDa protein isolated from corn seeds. It inhibits mammalian trypsin and, Factor XIIa (Hageman Factor) of the contact pathway of coagulation as well, as alpha-amylases from several insect species. Among the plasma, proteinases, CHFI specifically inhibits Factor XIIa without affecting the, activity of other coagulation proteinases. We have isolated CHFI from corn, and determined the crystallographic structure at 1.95 A resolution., Additionally, we have solved the structure of the recombinant protein, produced in Escherichia coli at 2.2 A resolution. The two proteins are, essentially identical. The proteinase binding loop is in the canonical, conformation for proteinase inhibitors. In an effort to understand, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9799488 (full description)]]
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<StructureSection load='1bfa' size='340' side='right'caption='[[1bfa]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1bfa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BFA FirstGlance]. <br>
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1BFA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Zea_mays Zea mays]]. Structure known Active Site: SLE. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BFA OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bfa OCA], [https://pdbe.org/1bfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bfa RCSB], [https://www.ebi.ac.uk/pdbsum/1bfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bfa ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structural determinants of the bifunctional corn Hageman factor inhibitor: x-ray crystal structure at 1.95 A resolution., Behnke CA, Yee VC, Trong IL, Pedersen LC, Stenkamp RE, Kim SS, Reeck GR, Teller DC, Biochemistry. 1998 Nov 3;37(44):15277-88. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9799488 9799488]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/ITRF_MAIZE ITRF_MAIZE] Potent inhibitor of mammalian trypsin and a specific inhibitor of factor XIIa (activated hageman factor).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/1bfa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bfa ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Zea mays]]
[[Category: Zea mays]]
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[[Category: Behnke, C.A.]]
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[[Category: Behnke CA]]
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[[Category: Kim, S.S.]]
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[[Category: Kim SS]]
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[[Category: Pedersen, L.C.]]
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[[Category: Le Trong I]]
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[[Category: Reeck, G.R.]]
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[[Category: Pedersen LC]]
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[[Category: Stenkamp, R.E.]]
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[[Category: Reeck GR]]
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[[Category: Teller, D.C.]]
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[[Category: Stenkamp RE]]
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[[Category: Trong, I.Le.]]
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[[Category: Teller DC]]
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[[Category: Yee, V.C.]]
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[[Category: Yee VC]]
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[[Category: amylase/protease bifunctional inhibitor]]
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[[Category: serine protease inhibitor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:54:33 2007''
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Current revision

RECOMBINANT BIFUNCTIONAL HAGEMAN FACTOR/AMYLASE INHIBITOR FROM MAIZE

PDB ID 1bfa

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