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1bgd

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(New page: 200px<br /><applet load="1bgd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bgd, resolution 2.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1bgd.gif|left|200px]]<br /><applet load="1bgd" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bgd, resolution 2.3&Aring;" />
 
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'''CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)==
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The crystal structures of recombinant canine and bovine granulocyte colony, stimulating factor (G-CSF) have been determined by X-ray crystallography, using molecular replacement with recombinant human G-CSF as a model. G-CSF, is a member of the cytokine family of glycoproteins that stimulate the, differentiation and proliferation of blood cells. Human, bovine and canine, G-CSF all have a molecular mass of about 19 kDa and share an amino acid, sequence identity of about 80%. Two crystal forms of canine G-CSF have, been solved. Form I recombinant canine G-CSF (rcG-CSFI; space group C2), contains one molecule in the asymmetric unit while form II canine G-CSF, (rcG-CSFII; space group P2(1)) has two molecules in the asymmetric unit, and bovine G-CSF (rbG-CSF; space group P2(1)2(1)2(1)) contains one, molecule in the asymmetric unit. rcG-CSFI has been refined to an R factor, of 20.7% with data to 2.3 A resolution and rcG-CSFII has been refined to, an R factor of 19.3% with data to 2.2 A resolution. rbG-CSF has been, refined to an R factor of 21.3% with data to 1.7 A resolution. The, structure of human, canine and bovine G-CSF is an antiparallel, 4-alpha-helical bundle with up-up-down-down connectivity. With the, exception of one highly exposed loop (residues 66 to 74), the human, canine and bovine structures are very similar to each other. Using our, series of G-CSF crystal structures we developed a function that describes, the probability that a particular residue position (i) contributes to a, G-CSF receptor binding site based on two principles, (1) high sequence, conservation in the primary sequence of human, bovine, canine and murine, G-CSF and (2) conservation of high solvent accessibility in the human, bovine and canine crystal structures. On the basis of this probability, function as well as a comparison of G-CSF to the crystal structure of, human growth hormone (hGH) complexed with the extracellular domain of the, human growth hormone receptor (hGHbp), residues that contribute to, potential G-CSF receptor binding sites are identified.
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<StructureSection load='1bgd' size='340' side='right'caption='[[1bgd]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bgd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BGD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bgd OCA], [https://pdbe.org/1bgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bgd RCSB], [https://www.ebi.ac.uk/pdbsum/1bgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bgd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CSF3_CANLF CSF3_CANLF] Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bg/1bgd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bgd ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BGD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BGD OCA].
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*[[Colony-stimulating factor 3D structures|Colony-stimulating factor 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of canine and bovine granulocyte-colony stimulating factor (G-CSF)., Lovejoy B, Cascio D, Eisenberg D, J Mol Biol. 1993 Dec 5;234(3):640-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7504736 7504736]
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[[Category: Canis lupus familiaris]]
[[Category: Canis lupus familiaris]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cascio, D.]]
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[[Category: Cascio D]]
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[[Category: Eisenberg, D.]]
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[[Category: Eisenberg D]]
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[[Category: Lovejoy, B.]]
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[[Category: Lovejoy B]]
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[[Category: cytokine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:37:58 2007''
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Current revision

CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)

PDB ID 1bgd

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