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1bj3

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[[Image:1bj3.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF COAGULATION FACTOR IX-BINDING PROTEIN (IX-BP) FROM VENOM OF HABU SNAKE WITH A HETERODIMER OF C-TYPE LECTIN DOMAINS==
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The line below this paragraph, containing "STRUCTURE_1bj3", creates the "Structure Box" on the page.
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<StructureSection load='1bj3' size='340' side='right'caption='[[1bj3]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1bj3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Protobothrops_flavoviridis Protobothrops flavoviridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BJ3 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_1bj3| PDB=1bj3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bj3 OCA], [https://pdbe.org/1bj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bj3 RCSB], [https://www.ebi.ac.uk/pdbsum/1bj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bj3 ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF COAGULATION FACTOR IX-BINDING PROTEIN (IX-BP) FROM VENOM OF HABU SNAKE WITH A HETERODIMER OF C-TYPE LECTIN DOMAINS'''
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== Function ==
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[https://www.uniprot.org/uniprot/SLA_PROFL SLA_PROFL] Anticoagulant protein which binds to the gamma-carboxyglutamic acid-domain regions of factor IX (F9) (but not factor X) in the presence of calcium with a 1 to 1 stoichiometry.<ref>PMID:12695512</ref> <ref>PMID:8749314</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Coagulation factor IX-binding protein (IX-bp) isolated from the venom of the habu snake (Trimeresurus flavoviridis) is a disulfide-linked heterodimer consisting of homologous subunits A and B. The structure of IX-bp has been solved by X-ray crystallography at 2.6 A resolution to a crystallographic R -value of 0.181. The main-chain fold of each subunit is homologous to the carbohydrate-recognition domain of C-type lectins (C-type CRDs) except for the extended central loop. The structure is almost identical with that of factors IX and X-binding protein (IX/X-bp) as expected from the high level of amino acid sequence homology. The functional difference in ligand recognition from IX/X-bp must reside in the amino acid differences. A continuity of different amino acid residues located from the C-terminal of the second alpha-helix to the following loop forms the local conformational difference in this region between the two proteins. This loop participates in the formation of the concave surface between the two subunits, the putative binding site for the Gla-domain (gamma-carboxyglutamic acid-containing domain) of the coagulation factors. Another difference between the two proteins is in the relative disposition of subunits A and B. When the B subunits are superimposed, about a 6 degrees rotation is required for the superposition of the A subunits. A calcium ion links the second alpha-helix region to the C-terminal tail in each subunit and helps to stabilize the structure for Gla-domain binding. The interface created by the central loop swapping in the dimer IX-bp is almost identical with that seen within the monomeric C-type CRDs. This dimer forms as the result of the amino acid deletion in the linker region of the central loop of the original C-type lectins. Such a dimerization disrupts the lectin active site and creates a Gla-domain binding site, imparting functional diversity.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/1bj3_consurf.spt"</scriptWhenChecked>
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1BJ3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Trimeresurus_flavoviridis Trimeresurus flavoviridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJ3 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of coagulation factor IX-binding protein from habu snake venom at 2.6 A: implication of central loop swapping based on deletion in the linker region., Mizuno H, Fujimoto Z, Koizumi M, Kano H, Atoda H, Morita T, J Mol Biol. 1999 May 28;289(1):103-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10339409 10339409]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bj3 ConSurf].
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[[Category: Protein complex]]
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<div style="clear:both"></div>
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[[Category: Trimeresurus flavoviridis]]
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== References ==
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[[Category: Atoda, H.]]
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<references/>
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[[Category: Fujimoto, Z.]]
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__TOC__
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[[Category: Kano, H.]]
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</StructureSection>
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[[Category: Koizumi, M.]]
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[[Category: Large Structures]]
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[[Category: Mizuno, H.]]
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[[Category: Protobothrops flavoviridis]]
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[[Category: Morita, T.]]
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[[Category: Atoda H]]
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[[Category: C-type lectin superfamily]]
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[[Category: Fujimoto Z]]
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[[Category: Coagulation factor ix-binding]]
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[[Category: Kano H]]
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[[Category: Habu snake]]
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[[Category: Koizumi M]]
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[[Category: Heterodimer]]
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[[Category: Mizuno H]]
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[[Category: Venom]]
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[[Category: Morita T]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:34:45 2008''
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Current revision

CRYSTAL STRUCTURE OF COAGULATION FACTOR IX-BINDING PROTEIN (IX-BP) FROM VENOM OF HABU SNAKE WITH A HETERODIMER OF C-TYPE LECTIN DOMAINS

PDB ID 1bj3

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