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1boi

From Proteopedia

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[[Image:1boi.gif|left|200px]]<br />
 
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<applet load="1boi" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1boi, resolution 2.2&Aring;" />
 
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'''N-TERMINALLY TRUNCATED RHODANESE'''<br />
 
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==Overview==
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==N-TERMINALLY TRUNCATED RHODANESE==
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The NH2-terminal sequence of rhodanese influences many of its properties, ranging from mitochondrial import to folding. Rhodanese truncated by &gt;9, residues is degraded in Escherichia coli. Mutant enzymes with lesser, truncations are recoverable and active, but they show altered active site, reactivities (Trevino, R. J., Tsalkova, T., Dramer, G., Hardesty, B., Chirgwin, J. M., and Horowitz, P. M. (1998) J. Biol. Chem. 273, 27841-27847), suggesting that the NH2-terminal sequence stabilizes the, overall structure. We tested aspects of the conformations of these, shortened species. Intrinsic and probe fluorescence showed that truncation, decreased stability and increased hydrophobic exposure, while near UV CD, suggested altered tertiary structure. Under native conditions, truncated, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10318804 (full description)]]
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<StructureSection load='1boi' size='340' side='right'caption='[[1boi]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1boi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1boi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1boi OCA], [https://pdbe.org/1boi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1boi RCSB], [https://www.ebi.ac.uk/pdbsum/1boi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1boi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/THTR_BOVIN THTR_BOVIN] Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA (By similarity). Formation of iron-sulfur complexes and cyanide detoxification. Binds molecular oxygen and sulfur.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1boi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1boi ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BOI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]]. Active as [[http://en.wikipedia.org/wiki/Thiosulfate_sulfurtransferase Thiosulfate sulfurtransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.1 2.8.1.1]]. Structure known Active Site: CT1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BOI OCA]].
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*[[Sulfurtransferase|Sulfurtransferase]]
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__TOC__
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==Reference==
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</StructureSection>
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NH2-terminal sequence truncation decreases the stability of bovine rhodanese, minimally perturbs its crystal structure, and enhances interaction with GroEL under native conditions., Trevino RJ, Gliubich F, Berni R, Cianci M, Chirgwin JM, Zanotti G, Horowitz PM, J Biol Chem. 1999 May 14;274(20):13938-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10318804 10318804]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Thiosulfate sulfurtransferase]]
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[[Category: Berni R]]
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[[Category: Berni, R.]]
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[[Category: Cianci M]]
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[[Category: Cianci, M.]]
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[[Category: Gliubich F]]
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[[Category: Gliubich, F.]]
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[[Category: Horowitz PM]]
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[[Category: Horowitz, P.M.]]
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[[Category: Trevino RJ]]
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[[Category: Trevino, R.J.]]
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[[Category: Zanotti G]]
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[[Category: Zanotti, G.]]
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[[Category: rhodanese]]
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[[Category: sulfurtransferase]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:56:21 2007''
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Current revision

N-TERMINALLY TRUNCATED RHODANESE

PDB ID 1boi

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