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1bu4

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[[Image:1bu4.gif|left|200px]]<br />
 
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<applet load="1bu4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bu4, resolution 1.9&Aring;" />
 
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'''RIBONUCLEASE 1 COMPLEX WITH 2'GMP'''<br />
 
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==Overview==
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==RIBONUCLEASE 1 COMPLEX WITH 2'GMP==
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We systematically analyzed the crystallographically determined water, molecules of all known structures of RNase T1 and compared them to the, ordered solvent in a large number of related microbial nucleases. To, assess the crystallographers' impact on the interpretation of the solvent, structure, we independently refined five validation structures from, diffraction data derived from five isomorphous crystals of RNase T1. We, also compared the positions of water molecules found in 11 published, isomorphous RNase T1 inhibitor complexes. These data suggest that the, positions of most of the waters located on the surface of a protein and, that are well-determined in the experimental electron density maps are, determined primarily by crystal packing forces. Water molecules with less, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10373011 (full description)]]
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<StructureSection load='1bu4' size='340' side='right'caption='[[1bu4]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bu4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BU4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2GP:GUANOSINE-2-MONOPHOSPHATE'>2GP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bu4 OCA], [https://pdbe.org/1bu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bu4 RCSB], [https://www.ebi.ac.uk/pdbsum/1bu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bu4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNT1_ASPOR RNT1_ASPOR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bu/1bu4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bu4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BU4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]] with CA and 2GP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]]. Structure known Active Sites: CAL and CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BU4 OCA]].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Conserved water molecules in a large family of microbial ribonucleases., Loris R, Langhorst U, De Vos S, Decanniere K, Bouckaert J, Maes D, Transue TR, Steyaert J, Proteins. 1999 Jul 1;36(1):117-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10373011 10373011]
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[[Category: Aspergillus oryzae]]
[[Category: Aspergillus oryzae]]
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[[Category: Ribonuclease T(1)]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Bouckaert J]]
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[[Category: Bouckaert, J.]]
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[[Category: Decanniere K]]
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[[Category: Decanniere, K.]]
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[[Category: Devos S]]
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[[Category: Devos, S.]]
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[[Category: Langhorst U]]
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[[Category: Langhorst, U.]]
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[[Category: Loris R]]
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[[Category: Loris, R.]]
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[[Category: Maes D]]
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[[Category: Maes, D.]]
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[[Category: Steyaert J]]
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[[Category: Steyaert, J.]]
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[[Category: Transue TR]]
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[[Category: Transue, T.R.]]
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[[Category: 2GP]]
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[[Category: CA]]
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[[Category: endoribonuclease]]
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[[Category: hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:57:35 2007''
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Current revision

RIBONUCLEASE 1 COMPLEX WITH 2'GMP

PDB ID 1bu4

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